growth hormone Active Protein | zfGH active protein

Zebrafish growth hormone

Cat. Num. AAA400097

• Gene Names: GH1; GH; GHN; GH-N; hGH-N; IGHD1B
• Purity: Greater than 99.0% as determined by:; (a) Gel filtration analysis.; (b) Analysis by reducing and non-reducing SDS-PAGE gel.; (c) Analysis by RP-HPLC.
• Synonyms: growth hormone; Zebrafish growth hormone; Zebrafish growth hormone (zfGH); zfGH active protein

• Host: E Coli
• Purity/Purification: Greater than 99.0% as determined by:; (a) Gel filtration analysis.; (b) Analysis by reducing and non-reducing SDS-PAGE gel.; (c) Analysis by RP-HPLC.
• Form/Format: White lyophilized (freeze-dried) powder.
• Sequence: The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Gln-Arg-Leu-Phe-Asn.
• Sequence Length: 217

• Solubility: It is recommended to reconstitute the lyophilized zfGH in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml and not more than 3 mg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
• Protein Content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.42 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0 This value is calculated by the DNAman program.
• Dimers and Aggregates: The purified zfGH (21K) consists of > 99% monomers as determined by gel-filtration chromatography.
• Endotoxin: Less than 0.05 ng/ug (IEU/ug) of zfGH (22 K).
• Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.5% NaHCO3 adjusted to pH 8.
• Biological Activity: zfGH is biologically active in PDF-P1 3B9 cells stably transfected with rabbit GH receptors.
• Preparation and Storage: Lyophilized zfGH although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution at > 0.1 mg/ml and filter sterilization zfGH can be stored at 4 degree C for several weeks. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is recomended. Please prevent freeze-thaw cycles.

Related Product Information for zfGH active protein

Recombinant zebrafish growth hormone (zbGH) produced in E.Coli (22 K) is a single, non-glycosylated, polypeptide chain containing 185 amino acids with an additional Ala at its N-terminus and having a molecular mass of 21187 Dalton. zfGH is purified by proprietary chromatographic techniques.

Product Categories/Family for zfGH active protein

Growth Hormones; prolactins; placental lactogens

NCBI and Uniprot Product Information

• NCBI GI #: 183157
• NCBI GeneID: 2688
• UniProt Accession #: P01241; Q14405; Q16631; Q5EB53; Q9HBZ1; Q9UMJ7; Q9UNL5; A6NEF6
• Molecular Weight: 20,201 Da
• NCBI Official Full Name: growth hormone
• NCBI Official Synonym Full Names: growth hormone 1
• NCBI Official Symbol: GH1
• NCBI Official Synonym Symbols: GH; GHN; GH-N; hGH-N; IGHD1B
• NCBI Protein Information: somatotropin; pituitary growth hormone
• UniProt Protein Name: Somatotropin
• Protein Family: Growth hormone
• UniProt Gene Name: GH1
• UniProt Synonym Gene Names: GH; GH-N
• UniProt Entry Name: SOMA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. [provided by RefSeq, Jul 2008]

Uniprot Description

GH: Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A); also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH. Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B); also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH. Defects in GH1 are the cause of Kowarski syndrome (KWKS); also known as pituitary dwarfism VI. Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2). IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy. Belongs to the somatotropin/prolactin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Hormone

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; growth hormone receptor binding; growth factor activity; prolactin receptor binding; hormone activity; metal ion binding

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of MAP kinase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of receptor internalization; positive regulation of JAK-STAT cascade; glucose transport; positive regulation of multicellular organism growth; JAK-STAT cascade; response to estradiol stimulus; positive regulation of tyrosine phosphorylation of Stat3 protein

Disease: Isolated Growth Hormone Deficiency, Type Ia; Isolated Growth Hormone Deficiency, Type Ib; Isolated Growth Hormone Deficiency, Type Ii; Kowarski Syndrome