vascular endothelial growth factor Active Protein | VEGF-165 active protein

Human vascular endothelial growth factor (VEGF-165)

Cat. Num. AAA400074

• Gene Names: VEGFA; VPF; VEGF; MVCD1
• Purity: Greater than 98.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Anion-exchange FPLC.; (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.; (d) Gel filtration chromatography under non denaturative conditions
• Synonyms: vascular endothelial growth factor; Human vascular endothelial growth factor (VEGF-165); Human vascular endothelial growth factor 165 (VEGF-165); VEGF-165 active protein

• Host: E. coli
• Purity/Purification: Greater than 98.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Anion-exchange FPLC.; (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.; (d) Gel filtration chromatography under non denaturative conditions
• Form/Format: White lyophilized (freeze-dried) powder.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Ala-Glu.
• Sequence Length: 191

• Solubility: It is recommended to reconstitute the lyophilized VEGF in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other buffered aqueous solutions containing carrier proteins.
• Protein Content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.287 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
• Dimers and Aggregates: Less than 1% as determined by gel filtration chromatography under non denaturative conditions
• Endotoxin: Less than 0.1 ng/ug (IEU/ug) of VEGF.
• Formulation: Recombinant VEGF was lyophilized from a concentrated (1mg/ml) solution containg 0.02% NaHCO3.
• Biological Activity: The biological activity is determined by the dose-dependent stimulation of the proliferation of Ba/F3-VEGFR2 cells.
• Preparation and Storage: Lyophilized VEGF although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution rHuVEGF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).; Please prevent freeze-thaw cycles.

Related Product Information for VEGF-165 active protein

Recombinant Human VEGF is a non-glycosylated dimer of polypeptide chain containing 165 amino acids and having a molecular mass of 38231 Dalton. Recombinant VEGF is purified by proprietary techniques.

Product Categories/Family for VEGF-165 active protein

Hormones; Cytokines and Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 181971
• NCBI GeneID: 7422
• UniProt Accession #: P15692; O60720; O75875; Q074Z4; Q16889; B5BU86; H0Y2S8; H0Y407; H0Y414; H0Y462; H0Y8N2; H3BLW7
• NCBI Official Full Name: vascular endothelial growth factor
• NCBI Official Synonym Full Names: vascular endothelial growth factor A
• NCBI Official Symbol: VEGFA
• NCBI Official Synonym Symbols: VPF; VEGF; MVCD1
• NCBI Protein Information: vascular endothelial growth factor A; vascular permeability factor
• UniProt Protein Name: Vascular endothelial growth factor A
• Protein Family: Vascular endothelial growth factor
• UniProt Gene Name: VEGFA
• UniProt Synonym Gene Names: VEGF; VEGF-A; VPF
• UniProt Entry Name: VEGFA_HUMAN

NCBI Description

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.