Tissue Inhibitor of Metalloprotease 2 Active Protein | TIMP2 active protein

Recombinant Human Tissue Inhibitor of Metalloprotease 2

Cat. Num. AAA146081

• Gene Names: TIMP2; DDC8; CSC-21K
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Tissue Inhibitor of Metalloprotease 2; Recombinant Human Tissue Inhibitor of Metalloprotease 2; TIMP2 Human; Tissue Inhibitor of Metalloprotease 2 Human Recombinant; Fibroblast growth factor 17; FGF-17; FGF17; FGF-13; HH20; TIMP2 active protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: TIMP2 protein was lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, with 3 % trehalose.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: CSCSPVHPQQ AFCNADVVIR AKAVSEKEVD SGNDIYGNPI KRIQYEIKQI KMFKGPEKDI EFIYTAPSSA VCGVSLDVGG KKEYLIAGKA EGDGKMHITL CDFIVPWDTL STTQKKSLNH RYQMGCECKI TRCPMIPCYI SSPDECLWMD WVTEKNINGH QAKFFACIKR SDGSCAWYRG AAPPKQEFLD IEDP
• Sequence Length: 220

• Solubility: It is recommended to reconstitute the lyophilized TIMP2 in sterile 18M?-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: Fully biologically active when compared to standard. The biologically active as determined by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2.
• Preparation and Storage: Lyophilized TIMP2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution TIMP2 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Related Product Information for TIMP2 active protein

Description: TIMP2 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 194 amino acids and having a molecular mass of 21.8kDa.

Introduction: TIMP2 belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases that take part in degradation of the extracellular matrix. Besides having an inhibitory role against metalloproteinases, the encoded protein has a exclusive part among TIMP family members in its capability to directly suppress the proliferation of endothelial cells. Consequently, the encoded protein is crucial to the conservation of tissue homeostasis by suppressing the production of quiescent tissues as an answer to angiogenic factors, and by inhibiting protease activity in tissues undergoing renovation of the extracellular matrix.

Product Categories/Family for TIMP2 active protein

Enzymes; Metalloprotease

NCBI and Uniprot Product Information

• NCBI GI #: 4507511
• NCBI GeneID: 7077
• NCBI Accession #: NP_003246.1
• NCBI GenBank Nucleotide #: NM_003255.4
• UniProt Accession #: P16035; Q16121; Q93006; Q9UDF7
• Molecular Weight: 24,399 Da
• NCBI Official Full Name: metalloproteinase inhibitor 2
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 2
• NCBI Official Symbol: TIMP2
• NCBI Official Synonym Symbols: DDC8; CSC-21K
• NCBI Protein Information: metalloproteinase inhibitor 2; tissue inhibitor of metalloproteinases 2
• UniProt Protein Name: Metalloproteinase inhibitor 2
• Protein Family: Metalloproteinase inhibitor
• UniProt Gene Name: TIMP2
• UniProt Synonym Gene Names: TIMP-2
• UniProt Entry Name: TIMP2_HUMAN

NCBI Description

This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008]

Uniprot Description

TIMP2: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. Interacts (via the C-terminal) with MMP2 (via the C- terminal PEX domain); the interaction inhibits the MMP2 activity. Down-regulated by TGFB1. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted; Inhibitor; Cell development/differentiation; Extracellular matrix

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: extracellular space; proteinaceous extracellular matrix; growth cone; cell surface; cell soma; extracellular region; basement membrane

Molecular Function: integrin binding; metalloendopeptidase inhibitor activity; protein binding; protease binding; metal ion binding; enzyme activator activity

Biological Process: regulation of Rap protein signal transduction; response to drug; negative regulation of metalloenzyme activity; extracellular matrix organization and biogenesis; central nervous system development; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; negative regulation of cell proliferation; extracellular matrix disassembly; positive regulation of MAPKKK cascade; response to cytokine stimulus; negative regulation of Ras protein signal transduction; negative regulation of mitotic cell cycle; positive regulation of adenylate cyclase activity; positive regulation of neuron differentiation; aging