Trefoil Factor-3 Active Protein | TFF3 active protein
Recombinant Human Trefoil Factor-3
Gene Names
TFF3; ITF; P1B; TFI
Purity
Greater than 97.0% as determined by SDS-PAGE.
Synonyms
Trefoil Factor-3; Recombinant Human Trefoil Factor-3; TFF3 Human; Trefoil Factor-3 Human Recombinant; TFF-3; ITF; TFI; HITF; hP1.B; TFF3; Trefoil factor 3; Intestinal trefoil factor; TFF3 active protein
Host
E Coli
Purity/Purification
Greater than 97.0% as determined by SDS-PAGE.
Form/Format
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Sequence
EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF
Sequence Length
94
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility
It is recommended to reconstitute the lyophilized TFF3 in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
Determined by its ability to chemoattract human MCF-7 cells using a concentration 1.0-10.0 ng/ml.
Preparation and Storage
Lyophilized TFF3 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution TFF3 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Related Product Information for TFF3 active protein
Description: TFF-3 Human Recombinant produced in E Coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 59 amino acid chains which includes a 40 amino acid trefoil motif containing 3 conserved interamolecular disulfide bonds and having a total molecular mass of 13.2kDa. TFF-3 Human Recombinant is purified by proprietary chromatographic techniques.
Introduction: Proteins of the TFF family are characterized by obtaining a minimum of 1 copy of the trefoil motif, a 40-amino acid domain that contains 3 conserved disulfides. Trefoil Factors are stable secretory proteins expressed in gastrointestinal mucosa which protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium.TFF2 inhibits gastric acid motility & secretion. TFF2 stabilizes glycoproteins in the mucus gel through interactions with carbohydrate side chains. TFF3 induces ciliogenesis and promotes airway epithelial ciliated cell differentiation, relatively through an epidermal growth factor receptor-dependent pathway. TFF3 overexpression is crucial for progression in mouse and human hepatocellular carcinogenesis. TFF-3 is normally expressed in hepatocellular carcinoma and its expression associates with tumor grade.
Introduction: Proteins of the TFF family are characterized by obtaining a minimum of 1 copy of the trefoil motif, a 40-amino acid domain that contains 3 conserved disulfides. Trefoil Factors are stable secretory proteins expressed in gastrointestinal mucosa which protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium.TFF2 inhibits gastric acid motility & secretion. TFF2 stabilizes glycoproteins in the mucus gel through interactions with carbohydrate side chains. TFF3 induces ciliogenesis and promotes airway epithelial ciliated cell differentiation, relatively through an epidermal growth factor receptor-dependent pathway. TFF3 overexpression is crucial for progression in mouse and human hepatocellular carcinogenesis. TFF-3 is normally expressed in hepatocellular carcinoma and its expression associates with tumor grade.
Product Categories/Family for TFF3 active protein
References
Quantitative electrochemical metalloimmunoassay for TFF3 in urine using a paper analytical device. Paul R. DeGregory, Yi-Ju Tsai, Karen Scida, Ian Richards and Richard M. Crooks (2016)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
8,641 Da
NCBI Official Full Name
trefoil factor 3
NCBI Official Synonym Full Names
trefoil factor 3 (intestinal)
NCBI Official Symbol
TFF3
NCBI Official Synonym Symbols
ITF; P1B; TFI
NCBI Protein Information
trefoil factor 3; polypeptide P1.B
UniProt Protein Name
Trefoil factor 3
Protein Family
UniProt Gene Name
TFF3
UniProt Synonym Gene Names
ITF; TFI; hITF; hP1.B
UniProt Entry Name
TFF3_HUMAN
NCBI Description
Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]
Uniprot Description
TFF3: Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: proteinaceous extracellular matrix; extracellular region; secretory granule
Biological Process: response to peptide hormone stimulus; digestion; defense response
Research Articles on TFF3
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Product Notes
The TFF3 tff3 (Catalog #AAA144182) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQE AECTF. It is sometimes possible for the material contained within the vial of "Trefoil Factor-3, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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