Neuregulin/Heregulin-1beta (NRG-1beta/HRG-1beta) Active Protein | SMDF active protein

Neuregulin / Heregulin-1beta (NRG-1beta / HRG-1beta), human recombinant

Cat. Num. AAA844847

• Gene Names: CTSG; CG; CATG
• Applications: SDS-Page
• Purity: >=98%
• Synonyms: Neuregulin/Heregulin-1beta (NRG-1beta/HRG-1beta); Neuregulin / Heregulin-1beta (NRG-1beta / HRG-1beta); human recombinant; NGR beta 1; Heregulin; HRG 1beta; Neuregulin; Neuregulin1 (NRG1-beta1); Neu differentiation factor; Pro-neuregulin-1; Acetylcholine receptor-inducing activity; Breast cancer cell differentiation factor p45; Glial growth factor; Sensory and motor neuron-derived factor; SMDF active protein

• Host: E Coli
• Purity/Purification: >=98%
• Form/Format: < 0.5 ng/ml; Appearance: Lyophilized protein
• Sequence Length: 255

• Applicable Applications for SMDF active protein: SDS-PAGE; HPLC
• Solubility/Reconstitution Instructions: Reconstitute in H?O to a concentration of 0.1-1.0 ug/ul. The solution can then be diluted into other aqueous buffers and stored at 4 degree C for 1 week or -20 degree C for future use.
• Endotoxin Level: <0.1 ng/mug
• Biological Activity: The ED?? was determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells is < 0.5 ng/ml, corresponding to a specific activity of > 2 x 10 units/mg.
• Results: <0.5 ng/ml
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for SMDF active protein

Background: Neuregulin/Heregulin is a family of structurally related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. Proteolytic processing of the extraCellular domain of the transmembrane NRG1 isoforms release soluble growth factors. HRG1-beta1 contains an Ig domain and an EGF-like domain that is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. This binding induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity, which leads to tyrosine phosphorylation. Although HRG1-beta1 biological effects is still unclear, it has been found to promote motility and invasiveness of breast cancer cells which may also involve up-regulation of expression and function of the autocrine motility-promoting factor (AMF). Recombinant human Heregulin-beta1 (HRG1-beta1) is a 7.5 kDa polypeptide consisting of only the EGF domain of heregulin-beta1 (65 amino acid residues).

NCBI and Uniprot Product Information

• NCBI GI #: 4503149
• NCBI GeneID: 1511
• NCBI Accession #: NP_001902.1
• NCBI GenBank Nucleotide #: NM_001911.2
• UniProt Accession #: P08311; Q6IBJ6; Q9UCA5; Q9UCU6
• Molecular Weight: 7.86 kDa
• NCBI Official Full Name: cathepsin G preproprotein
• NCBI Official Synonym Full Names: cathepsin G
• NCBI Official Symbol: CTSG
• NCBI Official Synonym Symbols: CG; CATG
• NCBI Protein Information: cathepsin G
• UniProt Protein Name: Cathepsin G
• UniProt Gene Name: CTSG
• UniProt Synonym Gene Names: CG
• UniProt Entry Name: CATG_HUMAN

NCBI Description

The protein encoded by this gene, a member of the peptidase S1 protein family, is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. In addition, the encoded protein is antimicrobial, with bacteriocidal activity against S. aureus and N. gonorrhoeae. Transcript variants utilizing alternative polyadenylation signals exist for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

CTSG: Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe- CH2Cl and phenylmethylsulfonyl fluoride. Belongs to the peptidase S1 family.

Protein type: Motility/polarity/chemotaxis; EC 3.4.21.20; Protease

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular matrix; extracellular space; cell surface; extracellular region; plasma membrane; nucleus; secretory granule

Molecular Function: peptidase activity; heparin binding; serine-type endopeptidase activity

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; positive regulation of immune response; cellular protein metabolic process; immune response; response to lipopolysaccharide; protein processing; proteolysis; angiotensin maturation; defense response to fungus