Stromal Derived Factor 1 alpha Active Protein | SDF1a active protein

Human Stromal Derived Factor 1 alpha (SDF1a)

Cat. Num. AAA400072

• Gene Names: CXCL12; IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12
• Purity: Greater than 98.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Anion-exchange FPLC.; (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.; (d) Gel fitration under non denaturative condition on Superdex 75 column.
• Synonyms: Stromal Derived Factor 1 alpha; Human Stromal Derived Factor 1 alpha (SDF1a); Human stromal derived factor 1 alpha; SDF1a active protein

• Host: E Coli
• Purity/Purification: Greater than 98.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Anion-exchange FPLC.; (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.; (d) Gel fitration under non denaturative condition on Superdex 75 column.
• Form/Format: Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Lys-Pro-Val-Ser-Leu
• Sequence Length: 89

• Solubility: It is recommended to reconstitute the lyophilized rHuSDF-1a in sterile 18Momega-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions, preferably with carrier protein.
• Protein Content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 1.06 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
• Dimers and Aggregates: Less than 1% as determined by gel filtration chromatography.
• Endotoxin: Less than 0.1 ng/ug (IEU/ug) of rHuSDF-1.
• Formulation: Lyophilized from a concentrated (1 mg/ml) solution in water containing no additives.
• Biological Activity: SDF-1a is fully biologically active when compared to standard. The specific activity as determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentation of 20-80 ng/ml.
• Preparation and Storage: Lyophilized rHuSDF-1a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuSDF-1a should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).; Please avoid freeze-thaw cycles.

Related Product Information for SDF1a active protein

Recombinant Human SDF-1 produced in E.Coli is a non-glycosylated, polypeptide chain containing 68 amino acids and having a molecular mass of 8008 Dalton. The rHuSDF-1a is purified by proprietary chromatographic techniques.

Product Categories/Family for SDF1a active protein

Hormones; Cytokines and Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 40316924
• NCBI GeneID: 6387
• NCBI Accession #: NP_954637.1
• NCBI GenBank Nucleotide #: NM_199168.3
• UniProt Accession #: P48061; Q2L985; Q2L986; Q2L988; Q5IT36; Q6ICW0; Q9H554; B2R4G0; E7EVL0; H7BYN8
• Molecular Weight: 11,004 Da
• NCBI Official Full Name: stromal cell-derived factor 1 isoform alpha
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 12
• NCBI Official Symbol: CXCL12
• NCBI Official Synonym Symbols: IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12
• NCBI Protein Information: stromal cell-derived factor 1; intercrine reduced in hepatomas; pre-B cell growth-stimulating factor
• UniProt Protein Name: Stromal cell-derived factor 1
• UniProt Gene Name: CXCL12
• UniProt Synonym Gene Names: SDF1; SDF1A; SDF1B; SDF-1; hSDF-1; IRH; hIRH; PBSF
• UniProt Entry Name: SDF1_HUMAN

NCBI Description

This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

CXCL12: Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to another C-X-C chemokine receptor CXCR7, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1- alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3- 67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and CXCR7, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T- cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of CXCR7 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Monomer or homodimer; in equilibrium. Dimer formation is induced by non acidic pH and the presence of multivalent anions, and by binding to CXCR4 or heparin. Monomeric form is required for full chemotactic activity and resistance to ischemia/reperfusion injury, whereas the dimeric form acts as a partial agonist of CXCR4, stimulating Ca2+ mobilization but with no chemotactic activity and instead acts as a selective antagonist that blocks chemotaxis induced by the monomeric form. Interacts with the N- terminus of CXCR7. Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen. Belongs to the intercrine alpha (chemokine CxC) family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Chemokine; Cell development/differentiation; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q11.1

Cellular Component: extracellular space; extracellular region; external side of plasma membrane

Molecular Function: growth factor activity; chemokine receptor binding; chemokine activity; CXCR chemokine receptor binding; receptor binding

Biological Process: positive regulation of dopamine secretion; response to peptide hormone stimulus; positive regulation of cell adhesion; adult locomotory behavior; blood circulation; neuron migration; motor axon guidance; signal transduction; chemotaxis; germ cell development; induction of positive chemotaxis; T cell proliferation; response to radiation; germ cell migration; cell adhesion; organ regeneration; response to virus; regulation of actin polymerization and/or depolymerization; cellular calcium ion homeostasis; patterning of blood vessels; G-protein coupled receptor protein signaling pathway; response to mechanical stimulus; response to heat; ameboidal cell migration; positive regulation of axon extension involved in axon guidance; response to hypoxia; positive regulation of endothelial cell proliferation; immune response; telencephalon cell migration

Disease: Human Immunodeficiency Virus Type 1, Susceptibility To