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Epidermal Growth Factor Active Protein | rHuEGF active protein

Recombinant Human Epidermal Growth Factor

Gene Names
EGF; URG; HOMG4
Purity
Greater than 98% by RP-HPLC, anion-exchange chromatography; reducing and non-reducing SDS-PAGE, silver stain.
Synonyms
Epidermal Growth Factor; Recombinant Human Epidermal Growth Factor; Human Epidermal Growth Factor (rHuEGF); rHuEGF active protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
Greater than 98% by RP-HPLC, anion-exchange chromatography; reducing and non-reducing SDS-PAGE, silver stain.
Form/Format
Sterile filtered, lyophilized
Sequence Length
53
Biological Activity
Fully biologically active compared to reference standards. Specific activity of 1 x 107 Units/mg.
Endotoxin
<0.1 ng/ug
Stabilizers
None
Preservatives
None
Protein Content
Determined spectrophotometrically at 280nm (2.858 ext. coeff), and by RP-HPLC analysis against a calibrated standard.
Dimers/Aggregates
<1% by SDS-PAGE
Reconstitution
Reconstitute the lyophilized product in sterile water not less than 100 ug/ml.
Preparation and Storage
Lyophilized product is stable at room temperature for 3 weeks.
Store lyophilized product desiccated at-20 degree C.
Upon reconstitution, store at 4 degree C for 2-7 days or aliquot and store at-20 degree C.
For long-term storage add either HSA or BSA to 0.1%.
Avoid repeated freeze-thaw cycles.
Related Product Information for rHuEGF active protein
Recombinant Human EGF produced in E Coli is a single chain, nonglycosylated polypeptide chain containing 53 amino acids with a molecular mass of 6,222 daltons. This product has full biological activity.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
129,256 Da
NCBI Official Full Name
epidermal growth factor, partial
NCBI Official Synonym Full Names
epidermal growth factor
NCBI Official Symbol
EGF
NCBI Official Synonym Symbols
URG; HOMG4
NCBI Protein Information
pro-epidermal growth factor
UniProt Protein Name
Pro-epidermal growth factor
Protein Family
UniProt Gene Name
EGF
UniProt Synonym Gene Names
EGF

NCBI Description

This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]

Uniprot Description

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).

Research Articles on rHuEGF

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Product Notes

The rHuEGF egf (Catalog #AAA191479) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Epidermal Growth Factor, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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