Fructose-2,6-bisphosphatase TIGAR Protein | TIGAR protein

Fructose-2,6-bisphosphatase TIGAR

• Gene Names: TIGAR; FR2BP; C12orf5
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: Fructose-2; 6-bisphosphatase TIGAR; C12orf5; TP53-induced glycolysis and apoptosis regulator; TP53-induced glycolysis regulatory phosphatase; TIGAR protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid
• Sequence Length: 270

• Applicable Applications for TIGAR protein: ELISA (EIA), Western Blot (WB), Affinity Purification (AP), Protein Array
• Tags: His
• Storage Buffer: 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole,10%glycerol(PH8.0)
• Preparation and Storage: Store at -20 degree C.; Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 9966849
• NCBI GeneID: 57103
• NCBI Accession #: NP_065108.1
• NCBI GenBank Nucleotide #: NM_020375.2
• UniProt Accession #: Q9NQ88; B2R840
• Molecular Weight: 30,063 Da
• NCBI Official Full Name: fructose-2,6-bisphosphatase TIGAR
• NCBI Official Synonym Full Names: TP53 induced glycolysis regulatory phosphatase
• NCBI Official Symbol: TIGAR
• NCBI Official Synonym Symbols: FR2BP; C12orf5
• NCBI Protein Information: fructose-2,6-bisphosphatase TIGAR
• UniProt Protein Name: Fructose-2,6-bisphosphatase TIGAR
• Protein Family: Fructose-2,6-bisphosphatase
• UniProt Gene Name: TIGAR

NCBI Description

This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region. [provided by RefSeq, Jul 2008]

Uniprot Description

Fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate as well as fructose-1,6-bisphosphate (PubMed:19015259). Acts as a negative regulator of glycolysis by lowering intracellular levels of fructose-2,6-bisphosphate in a p53/TP53-dependent manner, resulting in the pentose phosphate pathway (PPP) activation and NADPH production (PubMed:16839880, PubMed:22887998). Contributes to the generation of reduced glutathione to cause a decrease in intracellular reactive oxygen species (ROS) content, correlating with its ability to protect cells from oxidative or metabolic stress-induced cell death (PubMed:16839880, PubMed:19713938, PubMed:23726973, PubMed:22887998, PubMed:23817040). Plays a role in promoting protection against cell death during hypoxia by decreasing mitochondria ROS levels in a HK2-dependent manner through a mechanism that is independent of its fructose-bisphosphatase activity (PubMed:23185017). In response to cardiac damage stress, mediates p53-induced inhibition of myocyte mitophagy through ROS levels reduction and the subsequent inactivation of BNIP3. Reduced mitophagy results in an enhanced apoptotic myocyte cell death, and exacerbates cardiac damage (). Plays a role in adult intestinal regeneration; contributes to the growth, proliferation and survival of intestinal crypts following tissue ablation (PubMed:23726973). Plays a neuroprotective role against ischemic brain damage by enhancing PPP flux and preserving mitochondria functions (). Protects glioma cells from hypoxia- and ROS-induced cell death by inhibiting glycolysis and activating mitochondrial energy metabolism and oxygen consumption in a TKTL1-dependent and p53/TP53-independent manner (PubMed:22887998). Plays a role in cancer cell survival by promoting DNA repair through activating PPP flux in a CDK5-ATM-dependent signaling pathway during hypoxia and/or genome stress-induced DNA damage responses (PubMed:25928429). Involved in intestinal tumor progression (PubMed:23726973).