c-AMP dependant Protein Kinase A catalytic subunit alpha Active Protein | PRKACA active protein

Recombinant c-AMP dependant Protein Kinase A catalytic subunit alpha

Cat. Num. AAA142746

• Gene Names: PRKACA; PKACA; PPNAD4
• Purity: Greater than 95% as determined by SDS-PAGE.
• Synonyms: c-AMP dependant Protein Kinase A catalytic subunit alpha; Recombinant c-AMP dependant Protein Kinase A catalytic subunit alpha; PRKACA; cAMP-Dependent Protein Kinase A catalytic subunit alpha Recombinant; cAMP-dependent protein kinase alpha-catalytic subunit; EC 2.7.11.11; PKA C-alpha; PKACA; MGC48865; MGC102831; PRKACA active protein

• Host: E Coli
• Purity/Purification: Greater than 95% as determined by SDS-PAGE.
• Form/Format: PKA catalytic subunit a is supplied in a buffer containing 25mM potassium phosphate (pH 6.5), 5mM 2-mercaptoethanol, 5mM EDTA, 150mM NaCl and 50% glycerol.
• Sequence: MGNAAAAKKG SEQESVKEFL AKAKEDFLKK WESPAQNTAH LDQFERIKTL GTGSFGRVML VKHKETGNHY AMKILDKQKV VKLKQIEHTL NEKRILQAVN FPFLVKLEFS FKDNSNLYMV MEYVPGGEMF SHLRRIGRFS EPHARFYAAQ IVLTFEYLHS LDLIYRDLKP ENLLIDQQGY IQVTDFGFAK RVKGRTWTLC GTPEYLAPEI ILSKGYNKAV DWWALGVLIY EMAAGYPPFF ADQPIQIYEK IVSGKVRFPS HFSSDLKDLL RNLLQVDLTK RFGNLKNGVN DIKNHKWFAT TDWIAIYQRK VEAPFIPKFK GPGDTSNFDD YEEEEIRVSI NEKCGKEFSE F
• Sequence Length: 351

• Unit Defenition: One unit is defined as the amount of recombinant PKA catalytic subunit a, required to incorporate 1pmol of phosphate into the specific substrate peptide kemptide (LRRASlG) in one minute at 30 degree C.
• Specific Activity: The specific activity of the recombinant PKA catalytic subunit a, is >15.000.000 U/mg.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.

Related Product Information for PRKACA active protein

Description: cAMP-dependent PKA is an ubiquitous serine/threonine protein kinase present in a variety of tissues (e.g. brain, skeletal muscle, heart). The intracellular cAMP level regulates cellular responses by altering the interaction between the catalytic C and regulatory R subunits of PKA. The inactive tetrameric PKA holoenzyme R2C2 is activated when cAMP binds to R2, which dissociates the tetramer to R2 cAMP 4 and two active catalytic subunits. Free Catalytic subunits of PKA can phosphorylate a wide variety of intracellular target proteins. In response to hormone- induced high cAMP levels, PKA phosphorylates glycogen synthetase (inhibition of the enzyme activity) and phosphorylase kinase to block glycogen synthesis. Different isoforms of catalytic and regulatory subunits suggest specific functions. The recombinant PKA catalytic subunit a is a 41kDa protein. The a-isoform is the predominant form with a broad tissue distribution and can be used for in vitro enzymological studies of neural and hormonal signal transduction or to phosphorylate target proteins in vivo including Ion channels, transcriptional activator proteins and regulatory enzymes of glycogen metabolism.

Product Categories/Family for PRKACA active protein

Protein Kinases; Enzymes; Protein Kinase-A

NCBI and Uniprot Product Information

• NCBI GI #: 4506055
• NCBI GeneID: 5566
• NCBI Accession #: NP_002721.1
• NCBI GenBank Nucleotide #: NM_002730.3
• UniProt Accession #: P17612; Q32P54; Q9H2Y0; Q9NRB4; Q9NRH9
• Molecular Weight: 39,822 Da
• NCBI Official Full Name: cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha1
• NCBI Official Synonym Full Names: protein kinase, cAMP-dependent, catalytic, alpha
• NCBI Official Symbol: PRKACA
• NCBI Official Synonym Symbols: PKACA; PPNAD4
• NCBI Protein Information: cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; cAMP-dependent protein kinase catalytic subunit alpha, isoform 1; protein kinase A catalytic subunit
• UniProt Protein Name: cAMP-dependent protein kinase catalytic subunit alpha
• Protein Family: cAMP-dependent protein kinase
• UniProt Gene Name: PRKACA
• UniProt Synonym Gene Names: PKACA; PKA C-alpha
• UniProt Entry Name: KAPCA_HUMAN

NCBI Description

This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015]

Uniprot Description

PKACA: catalytic subunit of cAMP-dependent protein kinase alpha, an AGC kinase. A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Two splice-variant isoforms have been described.

Protein type: Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.11; AGC group; PKA family

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: centrosome; mitochondrion; membrane; plasma membrane; nucleus; neuromuscular junction; cytosol; cAMP-dependent protein kinase complex; AMP-activated protein kinase complex

Molecular Function: protein binding; cAMP-dependent protein kinase activity; ubiquitin protein ligase binding; protein serine/threonine/tyrosine kinase activity; protein kinase binding; ATP binding

Biological Process: nerve growth factor receptor signaling pathway; water transport; protein amino acid autophosphorylation; pathogenesis; signal transduction; protein amino acid phosphorylation; positive regulation of protein export from nucleus; triacylglycerol catabolic process; G2/M transition of mitotic cell cycle; transmembrane transport; sperm capacitation; epidermal growth factor receptor signaling pathway; regulation of synaptic transmission; cytosolic calcium ion homeostasis; fibroblast growth factor receptor signaling pathway; regulation of osteoblast differentiation; regulation of heart rate; organelle organization and biogenesis; activation of protein kinase A; peptidyl-threonine phosphorylation; glucose metabolic process; gluconeogenesis; peptidyl-serine phosphorylation; mesoderm formation; phospholipase C activation; regulation of protein binding; carbohydrate metabolic process; neural tube closure; energy reserve metabolic process; renal water homeostasis; innate immune response; mitotic cell cycle; blood coagulation; regulation of insulin secretion