placental growth hormone Active Protein | oplGH active protein

Ovine placental growth hormone

Cat. Num. AAA400051

• Gene Names: GH2; GHL; GHV; GH-V; hGH-V
• Purity: Greater than 98.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 150 nM NaCl, pH 8.; (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
• Synonyms: placental growth hormone; Ovine placental growth hormone; Ovine placental growth hormone (oplGH); oplGH active protein

• Host: E Coli
• Purity/Purification: Greater than 98.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 150 nM NaCl, pH 8.; (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
• Form/Format: Lyophylized white powder
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Phe-Pro-Ala, Less than 4% as determined by gel filtration chromatography
• Sequence Length: 245

• Solubility: It is recommended to reconstitute the lyophilized oGH in 0.4% NaHCO3 or water adjusted to pH 9, not less than 100ug/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.
• Protein Content: GH.Protein quantitation was carried out by two independent methods:; 1. UV spectroscopy at 280 nm using the absorbency value of 0.684 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).; 2. Analysis by RP-HPLC, using a calibrated solution of GH-22K-pl as a Reference Standard.
• Dimers and Aggregates: Less than 4% as determined by gel filtration chromatography
• Endotoxin: Less than 0.1 ng/ug (IEU/ug) of oplGH (22 K).
• Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3 previously adjusted pH 9.
• Biological Activity: oplGH is fully biologically active when compared to World Health Organization (WHO) reference standard using in vitro bioassay in PDF-P1 3B9 cells stably transfected with rabbit GH receptors. It is also capable of forming a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD).
• Preparation and Storage: Lyophilized oplGH although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution and filter sterilization oGH can be stored at 4 degree C, pH 9 for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Related Product Information for oplGH active protein

Recombinant ovine GH-22K produced in E. coli is a single, non-glycosylated, polypeptide chain containing 191 amino acids and having a molecular mass of 21980 Dalton. Predicted pI=7.81. oGH is purified by proprietary chromatographic techniques. It differs from pituitary oGH by two amino acids G9R/G63S and possesses higher biological activity as compared to pituitary oGH. For ref see Reicher et al. (submitted).

Product Categories/Family for oplGH active protein

Growth Hormones; prolactins; placental lactogens

NCBI and Uniprot Product Information

• NCBI GI #: 2459884
• NCBI GeneID: 2689
• UniProt Accession #: P01242; O14643; O14644; P09587; B1A4H5; B1A4H7
• Molecular Weight: 27,101 Da
• NCBI Official Full Name: placental growth hormone isoform hGH-V3
• NCBI Official Synonym Full Names: growth hormone 2
• NCBI Official Symbol: GH2
• NCBI Official Synonym Symbols: GHL; GHV; GH-V; hGH-V
• NCBI Protein Information: growth hormone variant; placenta-specific growth hormone; placental-specific growth hormone
• UniProt Protein Name: Growth hormone variant
• UniProt Gene Name: GH2
• UniProt Synonym Gene Names: GH-V
• UniProt Entry Name: SOM2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. As in the case of its pituitary counterpart, growth hormone 1, the predominant isoform of this particular family member shows similar somatogenic activity, with reduced lactogenic activity. Mutations in this gene lead to placental growth hormone/lactogen deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

GH2: Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. Belongs to the somatotropin/prolactin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: extracellular region

Molecular Function: hormone activity