TNF-Related Apoptosis Inducing Ligand/Apo2L Active Protein | mTRAIL active protein
Recombinant Mouse TNF-Related Apoptosis Inducing Ligand/Apo2L
Gene Names
Tnfsf10; TL2; Ly81; Trail; APO-2L; AI448571; A330042I21Rik
Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Synonyms
TNF-Related Apoptosis Inducing Ligand/Apo2L; Recombinant Mouse TNF-Related Apoptosis Inducing Ligand/Apo2L; TRAIL Mouse; TNF-Related Apoptosis Inducing Ligand/Apo2L Mouse Recombinant; Tumor necrosis factor ligand superfamily member 10; TNF-related apoptosis-inducing ligand; Protein TRAIL; Apo-2 ligand; Apo-2L; CD253 antigen; TL2; APO2L; TNFSF10; mTRAIL active protein
Host
E Coli
Purity/Purification
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Form/Format
The protein was lyophilized containing PBS, pH 7.4, and 3mM DTT.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sequence
MPRGGRPQKV AAHITGITRR SNSALIPISK DGKTLGQKIE SWESSRKGHS FLNHVLFRNG ELVIEQEGLY YIYSQTYFRF QEAEDASKMV SKDKVRTKQL VQYIYKYTSY PDPIVLMKSA RNSCWSRDAE YGLYSIYQGG LFELKKNDRI FVSVTNEHLM DLDQEASFFG AFLIN
Sequence Length
291
Solubility
It is recommended to reconstitute the lyophilized TRAIL Mouse Recombinant in sterile 18M?-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2,000,000 IU/mg in the presence of actinomycin D.
Preparation and Storage
Lyophilized TRAIL although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TRAIL recombinant should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Related Product Information for mTRAIL active protein
Description: TRAIL Recombinant Mouse produced in E Coli is a single, non-glycosylated polypeptide chain containing 175 amino acids and having a molecular mass of 20.2kDa.
Introduction: TNF-related apoptosis-inducing ligand (TRAIL) is a ligand molecule which induces apoptosis. It is a type II transmembrane protein with homology to other members of the tumor necrosis factor family.In humans, the gene that encodes for TRAIL is located at chromosome 3q26. TRAIL binds to the death receptors, DR4 and DR5. The process of apoptosis is caspase-8-dependent. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues.
Introduction: TNF-related apoptosis-inducing ligand (TRAIL) is a ligand molecule which induces apoptosis. It is a type II transmembrane protein with homology to other members of the tumor necrosis factor family.In humans, the gene that encodes for TRAIL is located at chromosome 3q26. TRAIL binds to the death receptors, DR4 and DR5. The process of apoptosis is caspase-8-dependent. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues.
Product Categories/Family for mTRAIL active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,477 Da
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 10
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 10
NCBI Official Symbol
Tnfsf10
NCBI Official Synonym Symbols
TL2; Ly81; Trail; APO-2L; AI448571; A330042I21Rik
NCBI Protein Information
tumor necrosis factor ligand superfamily member 10; TNF-related apoptosis inducing ligand; TNF-related apoptosis-inducing ligand; TRAIL/APO2L
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 10
UniProt Gene Name
Tnfsf10
UniProt Synonym Gene Names
Trail; Protein TRAIL
UniProt Entry Name
TNF10_MOUSE
Uniprot Description
TRAIL: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. Homotrimer. Widespread; most predominant in spleen, lung and prostate. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Cytokine
Cellular Component: extracellular space; membrane; integral to membrane
Molecular Function: cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding
Biological Process: apoptosis; positive regulation of apoptosis; positive regulation of caspase activity; immune response
Research Articles on mTRAIL
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Product Notes
The mTRAIL tnfsf10 (Catalog #AAA145539) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MPRGGRPQKV AAHITGITRR SNSALIPISK DGKTLGQKIE SWESSRKGHS FLNHVLFRNG ELVIEQEGLY YIYSQTYFRF QEAEDASKMV SKDKVRTKQL VQYIYKYTSY PDPIVLMKSA RNSCWSRDAE YGLYSIYQGG LFELKKNDRI FVSVTNEHLM DLDQEASFFG AFLIN ;. It is sometimes possible for the material contained within the vial of "TNF-Related Apoptosis Inducing Ligand/Apo2L, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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