Soluble RANK Ligand Active Protein | msRANKL active protein

Recombinant Mouse Soluble RANK Ligand

Cat. Num. AAA142197

• Gene Names: Tnfsf11; ODF; OPG; OPGL; RANKL; Ly109l; Trance
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: Soluble RANK Ligand; Recombinant Mouse Soluble RANK Ligand; sRANKL Mouse; RANK Ligand Soluble Mouse Recombinant; Soluble Receptor Activator of NFkB Ligand; TNFSF11; TRANCE; TNF-related activation-induced cytokine; OPGL; ODF; Osteoclast differentiation factor; Tumor necrosis factor ligand superfamily member 11; Receptor activator of nuclear factor kappa B ligand; RANKL; Osteoprotegerin ligand; CD254 antigen; sRANKL; sOdf; msRANKL active protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: The protein (1mg/ml) was lyophilized with 10mM Na2PO4, pH 7.5 & 50mM NaCl.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: MKPEAQPFAH LTINAASIPS GSHKVTLSSW YHDRGWAKIS NMTLSNGKLR VNQDGFYYLY ANICFRHHET SGSVPTDYLQ LMVYVVKTSI KIPSSHNLMK GGSTKNWSGN SEFHFYSINV GGFFKLRAGE EISIQVSNPS LLDPDQDATY FGAFKVQDID
• Sequence Length: 316

• Solubility: It is recommended to reconstitute the lyophilized sRANKL in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: Measured by its ability to induce osteoclast formation on murine RAW264.7 cells using a concentration of 50ng/ml shown in œCorning Osteo Assay Surface 24 Well Plates with Transwell Permeable Supports- A Useful Tool for Co-Culture Studies by Rebecca M. Wood and Mark Rothenber, corresponding to a specific activity of 20,000Units/mg.
• Preparation and Storage: Lyophilized TNFSF11 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution sRANKL should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for msRANKL active protein

Description: sRANKL Mouse Recombinant produced in E Coli is single, non-glycosylated, polypeptide chain containing 174 amino acids and having a total molecular mass of 19.9kDa.CD254 is purified by proprietary chromatographic techniques.

Introduction: RANKL binds to tnfrsf11b/opg and to tnfrsf11a/rank. Osteoclast differentiation and activation factor. augments the ability of dendritic cells to stimulate naive t-cell proliferation. May be an important regulator of interactions between t-cells and dendritic cells and may play a role in the regulation of the t-cell-dependent immune response. sRANKL may also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

Product Categories/Family for msRANKL active protein

Cytokines and Growth Factors; Growth Factors; RANK Ligand

NCBI and Uniprot Product Information

• NCBI GI #: 114842415
• NCBI GeneID: 21943
• NCBI Accession #: NP_035743.2
• NCBI GenBank Nucleotide #: NM_011613.3
• UniProt Accession #: O35235; O35306; Q3TWY5; Q9JJK8; Q9JJK9; Q9R1Y0
• Molecular Weight: 22,150 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 11
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 11
• NCBI Official Symbol: Tnfsf11
• NCBI Official Synonym Symbols: ODF; OPG; OPGL; RANKL; Ly109l; Trance
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 11; OPG ligand; TNF-related activation-induced cytokine; osteoclast differentiation factor; osteoprotegerin ligand; receptor activator of NF-kappaB ligand; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; tumor necrosis factor-related activation-induced cytokine
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 11
• UniProt Gene Name: Tnfsf11
• UniProt Synonym Gene Names: Opgl; Rankl; Trance; ODF; OPGL; RANKL; TRANCE
• UniProt Entry Name: TNF11_MOUSE

Uniprot Description

TNFSF11: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Homotrimer. Up-regulated by T-cell receptor stimulation. Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. Belongs to the tumor necrosis factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: extracellular space; membrane; cytoplasm; plasma membrane; integral to membrane; extracellular region; intracellular

Molecular Function: protein binding; cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding

Biological Process: positive regulation of osteoclast differentiation; multicellular organismal development; positive regulation of JNK cascade; osteoclast differentiation; activation of JNK activity; activation of NF-kappaB transcription factor; positive regulation of homotypic cell-cell adhesion; positive regulation of corticotropin-releasing hormone secretion; calcium ion homeostasis; positive regulation of MAP kinase activity; monocyte chemotaxis; regulation of osteoclast differentiation; tumor necrosis factor-mediated signaling pathway; positive regulation of bone resorption; cell differentiation; protein homooligomerization; bone resorption; ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; cytokine and chemokine mediated signaling pathway; mammary gland epithelial cell proliferation; lymph node development; positive regulation of protein kinase B signaling cascade; organ morphogenesis; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of T cell activation; positive regulation of phosphorylation