IFN-gamma active protein

Mouse IFN-gamma

Cat. Num. AAA691575

• Gene Names: Ifng; Ifg; IFN-g
• Reactivity: Mouse
• Purity: > 98% by SDS-PAGE & HPLC analyses
• Synonyms: IFN-gamma; Mouse IFN-gamma; Recombinant Murine IFN-gamma; Ifng; Ifg; IFN-g; IFN-gamma active protein

• Host: E Coli
• Reactivity: Mouse
• Purity/Purification: > 98% by SDS-PAGE & HPLC analyses
• Form/Format: Lyophilized
• Sequence: MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQ SQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD A FMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC
• Sequence Length: 134

• Endotoxin Level: < 0.1 ng/ug of protein (<1EU/ug)
• Biological Activity: Determined by its ability to inhibit the proliferation of murine WEHI-279 cells. The expected ED50 is <= 0.2 ng/ml, corresponding to a specific activity of >= 5 x 10^6 units/mg.
• Length: 134
• Reconstitution: Centrifuge vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/m. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Preparation and Storage: The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 12 months at -20°C to -80°C.

Related Product Information for IFN-gamma active protein

IFN-gamma is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-gamma receptors are present in most immune cells, which respond to IFN-gamma signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-gamma signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulates the antigen-specific phases of the immune response. Additionally, IFN-gamma stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-gamma species-specific and is biologically active only in human and primate cells. Recombinant murine IFN-gamma is a 15.6 kDa protein containing 134 amino acid residues.

Product Categories/Family for IFN-gamma active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 33468859
• NCBI GeneID: 15978
• NCBI Accession #: NP_032363.1
• NCBI GenBank Nucleotide #: NM_008337.3
• UniProt Accession #: P01580; Q542B8
• Molecular Weight: 15.6 kDa
• NCBI Official Full Name: interferon gamma
• NCBI Official Synonym Full Names: interferon gamma
• NCBI Official Symbol: Ifng
• NCBI Official Synonym Symbols: Ifg; IFN-g
• NCBI Protein Information: interferon gamma; IFN-gamma; gamma interferon
• UniProt Protein Name: Interferon gamma
• UniProt Gene Name: Ifng
• UniProt Synonym Gene Names: IFN-gamma
• UniProt Entry Name: IFNG_MOUSE

NCBI Description

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015]

Uniprot Description

Function: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Subunit structure: Homodimer.

Subcellular location: Secreted.

Tissue specificity: Released primarily from activated T lymphocytes.

Sequence similarities: Belongs to the type II (or gamma) interferon family.