Interferon-gamma Active Protein | mIFNG active protein

Recombinant Mouse Interferon-gamma

Cat. Num. AAA142235

• Gene Names: Ifng; Ifg; IFN-g
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Interferon-gamma; Recombinant Mouse Interferon-gamma; IFN g Mouse; Interferon-Gamma Mouse Recombinant; Immune Interferon; type II interferon; T cell interferon; MAF; IFNG; IFG; IFI; IFN-gamma; mIFNG active protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 um filtered concentrated (1mg/ml) solution in PBS, pH 7.4 and 5% trehalose.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC
• Sequence Length: 155

• Solubility: It is recommended to reconstitute the lyophilized Interferon-gamma in sterile distilled water or 20mM AcOH at concentrations ranging between 0.1mg-0.5mg/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The specific activity as determined in a viral resistance assay is < 0.1 ng/ml, corresponding to a specific activity of 1 x 107 Units/mg.
• Preparation and Storage: Lyophilized Interferon-gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IFN-gamma should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for mIFNG active protein

Description: Interferon-gamma Mouse Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 134 amino acids and having a molecular mass of 15.6kDa.The IFN-gamma is purified by proprietary chromatographic techniques.

Introduction: IFN-gamma produced by lymphocytes activated by specific antigens or mitogens.IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Product Categories/Family for mIFNG active protein

Cytokines and Growth Factors; Cytokines; Interferon

References

Title:Interferon-gamma and interleukin-4 reciprocally regulate CD8 expression in CD8+ T cells.Publication:Published online before print November 6, 2008, doi: 10.1073/pnas.0809549105 PNAS November 11, 2008 vol. 105 no. 45 17475-17480 Link:http://www.pnas.org/content/105/45/17475.full

NCBI and Uniprot Product Information

• NCBI GI #: 33468859
• NCBI GeneID: 15978
• NCBI Accession #: NP_032363.1
• NCBI GenBank Nucleotide #: NM_008337.3
• UniProt Accession #: P01580; Q542B8
• Molecular Weight: 17,907 Da
• NCBI Official Full Name: interferon gamma
• NCBI Official Synonym Full Names: interferon gamma
• NCBI Official Symbol: Ifng
• NCBI Official Synonym Symbols: Ifg; IFN-g
• NCBI Protein Information: interferon gamma; IFN-gamma; gamma interferon
• UniProt Protein Name: Interferon gamma
• UniProt Gene Name: Ifng
• UniProt Synonym Gene Names: IFN-gamma
• UniProt Entry Name: IFNG_MOUSE

NCBI Description

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015]

Uniprot Description

IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family.

Protein type: Secreted; Cytokine; Membrane protein, integral; Secreted, signal peptide

Cellular Component: extracellular space; cell; extracellular region; intracellular; external side of plasma membrane

Molecular Function: protein binding; interferon-gamma receptor binding; cytokine activity

Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; positive regulation of cell adhesion; positive regulation of osteoclast differentiation; positive regulation of interleukin-23 production; positive regulation of transcription, DNA-dependent; negative regulation of epithelial cell differentiation; positive regulation of interleukin-1 beta secretion; positive regulation of killing of cells of another organism; positive regulation of membrane protein ectodomain proteolysis; regulation of growth; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; positive regulation of synaptic transmission, cholinergic; neutrophil chemotaxis; CD8-positive, alpha-beta T cell differentiation during immune response; negative regulation of myelination; response to virus; defense response to protozoan; positive regulation of tumor necrosis factor production; inflammatory cell apoptosis; positive regulation of tyrosine phosphorylation of Stat1 protein; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-12 production; apoptosis; negative regulation of smooth muscle cell proliferation; positive regulation of interleukin-6 biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; sensory perception of mechanical stimulus; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; adaptive immune response; regulation of immune response; unfolded protein response; humoral immune response; antigen processing and presentation; positive regulation of chemokine biosynthetic process; positive regulation of interleukin-12 biosynthetic process; negative regulation of interleukin-17 production; protein import into nucleus, translocation; immune response; positive regulation of neuron differentiation; regulation of insulin secretion