Monoglyceride Lipase Active Protein | MGLL active protein

Recombinant Human Monoglyceride Lipase, Active

Cat. Num. AAA146830

• Gene Names: MGLL; MGL; HUK5; MAGL; HU-K5
• Purity: Greater than 85.0% as determined by SDS-PAGE.
• Synonyms: Monoglyceride Lipase; Recombinant Human Monoglyceride Lipase; Active; MGLL Human; Monoglyceride lipase; MGL; HU-K5; Lysophospholipase homolog; Lysophospholipase-like; Monoacylglycerol lipase; MAGL; MGLL; HUK5; Monoglyceride Lipase Human Recombinant; MGLL active protein

• Host: E Coli
• Purity/Purification: Greater than 85.0% as determined by SDS-PAGE.
• Form/Format: Sterile filtered colorless solution.; The MGLL solution (0.5mg/ml) contains 20mM Tris-HCl Buffer (pH 8.0) and 10% Glycerol.
• Sequence: MGSSHHHHHH SSGLVPRGSHMETGPEDPSS MPEESSPRRT PQSIPYQDLP HLVNADGQYL FCRYWKPTGT PKALIFVSHG AGEHSGRYEE LARMLMGLDLLVFAHDHVGH GQSEGERMVV SDFHVFVRDV LQHVDSMQKD YPGLPVFLLG HSMGGAIAIL TAAERPGHFAGMVLISPLVL ANPESATTFK VLAAKVLNLV LPNLSLGPID SSVLSRNKTE VDIYNSDPLI CRAGLKVCFGIQLLNAVSRV ERALPKLTVP FLLLQGSADR LCDSKGAYLL MELAKSQDKT LKIYEGAYHV LHKELPEVTNSVFHEINMWV SQRTATAGTA SPP.
• Sequence Length: 303

• Biological Activity: Specific activity is > 170 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl butyrate to pnitrophenol per minute at pH 7.5 at 25C.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for MGLL active protein

Description: MGLL Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 333 amino acids (1-313 a.a.) and having a molecular mass of 36.4kDa. The MGLL is purified by proprietary chromatographic techniques.
Introduction: MGLL is a membrane-associated member of the serine hydrolase superfamily. MGLL is expressed in abundance in skeletal muscle and adipose tissue. MGLL functions jointly with hormone-sensitive lipase (LIPE) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides.

Product Categories/Family for MGLL active protein

Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 51242953
• NCBI GeneID: 11343
• NCBI Accession #: NP_001003794.1
• NCBI GenBank Nucleotide #: NM_001003794.2
• UniProt Accession #: Q99685; Q6IBG9; Q96AA5; B3KRC2; B7Z9D1
• Molecular Weight: 31,149 Da
• NCBI Official Full Name: monoglyceride lipase isoform 2
• NCBI Official Synonym Full Names: monoglyceride lipase
• NCBI Official Symbol: MGLL
• NCBI Official Synonym Symbols: MGL; HUK5; MAGL; HU-K5
• NCBI Protein Information: monoglyceride lipase
• UniProt Protein Name: Monoglyceride lipase
• Protein Family: Monoglyceride lipase
• UniProt Gene Name: MGLL
• UniProt Synonym Gene Names: MAGL

NCBI Description

This gene encodes a serine hydrolase of the AB hydrolase superfamily that catalyzes the conversion of monoacylglycerides to free fatty acids and glycerol. The encoded protein plays a critical role in several physiological processes including pain and nociperception through hydrolysis of the endocannabinoid 2-arachidonoylglycerol. Expression of this gene may play a role in cancer tumorigenesis and metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

MGLL: Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain. Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth. Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family.

Protein type: EC 3.1.1.23; Lipid Metabolism - glycerolipid; Phospholipase

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: cytosol; endoplasmic reticulum membrane; extrinsic to membrane; membrane; nucleoplasm; plasma membrane

Molecular Function: acylglycerol lipase activity; lysophospholipase activity; protein homodimerization activity

Biological Process: acylglycerol catabolic process; arachidonic acid metabolic process; inflammatory response; lipid metabolic process; regulation of inflammatory response; regulation of sensory perception of pain; regulation of signal transduction