Fibroblast Growth Factor-8 Active Protein | mFGF 8 active protein

Recombinant Mouse Fibroblast Growth Factor-8

Cat. Num. AAA144417

• Gene Names: Fgf8; Aigf; Fgf-8
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Fibroblast Growth Factor-8; Recombinant Mouse Fibroblast Growth Factor-8; FGF 8 Mouse; Fibroblast Growth Factor-8 Mouse Recombinant; Fibroblast growth factor 8; FGF-8; Androgen-induced growth factor; AIGF; Heparin-binding growth factor 8; HBGF-8; Fgf8; mFGF 8 active protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: FGF-8 protein was lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: QVRSAAQKRG PGAGNPADTL GQGHEDRPFG QRSRAGKNFT NPAPNYPEEG SKEQRDSVLP KVTQRHVREQ SLVTDQLSRR LIRTYQLYSR TSGKHVQVLA NKRINAMAED GDPFAKLIVE TDTFGSRVRV RGAETGLYIC MNKKGKLIAK SNGKGKDCVF TEIVLENNYT ALQNAKYEGW YMAFTRKGRP RKGSKTRQHQ REVHFMKRLP RGHHTTEQSL RFEFLNYPPF TRSLRGSQRT WAPEPR.
• Sequence Length: 215

• Solubility: It is recommended to reconstitute the lyophilized FGF-8 in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The ED50, as determined by the dose-dependent a cell proliferation assay using NR6R-3T3 mouse fibroblast cells is <25 ng/ml in the presence of 0.1 ug/ml heprin, corresponding to a specific activity of > 4.0x104 units/mg.
• Preparation and Storage: Lyophilized FGF-8 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution FGF-8 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Related Product Information for mFGF 8 active protein

Description: FGF-8 Mouse Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 246 amino acids and having a molecular mass of 28.1kDa.The FGF-8 is purified by proprietary chromatographic techniques.

Introduction: FGF8 is part of the fibroblast growth factor family. FGF family members have wide mitogenic and cell survival activities, and participate in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF8 supports androgen and anchorage independent growth of mammary tumor cells. FGF8 over expression increases tumor growth and angiogensis. The adult expression of FGF-8 gene is restricted to testes and ovaries. FGF8 functions as an embryonic epithelial factor. FGF8 takes part in midbrain and limb development, organogenesis, embryo gastrulation and left-right axis determination.

Product Categories/Family for mFGF 8 active protein

Cytokines and Growth Factors; Growth Factors; Fibroblast Growth Factor

NCBI and Uniprot Product Information

• NCBI GI #: 261599076
• NCBI GeneID: 14179
• NCBI Accession #: NP_001159834.1
• NCBI GenBank Nucleotide #: NM_001166362.1
• UniProt Accession #: P37237
• Molecular Weight: 23,522 Da
• NCBI Official Full Name: fibroblast growth factor 8 isoform 3
• NCBI Official Synonym Full Names: fibroblast growth factor 8
• NCBI Official Symbol: Fgf8
• NCBI Official Synonym Symbols: Aigf; Fgf-8
• NCBI Protein Information: fibroblast growth factor 8; HBGF-8; androgen-induced growth factor; heparin-binding growth factor 8
• UniProt Protein Name: Fibroblast growth factor 8
• UniProt Gene Name: Fgf8
• UniProt Synonym Gene Names: Aigf; FGF-8; AIGF; HBGF-8
• UniProt Entry Name: FGF8_MOUSE

Uniprot Description

FGF8: Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Defects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6). Kallmann syndrome is a disorder that associates hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone- synthesizing neurons. In some patients other developmental anomalies can be present, which include renal agenesis, cleft lip and/or palate, selective tooth agenesis, and bimanual synkinesis. In some cases anosmia may be absent or inconspicuous. Defects in FGF8 are a cause of idiopathic hypogonadotropic hypogonadism (IHH). IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function. Belongs to the heparin-binding growth factors family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Cellular Component: extracellular space; extracellular region; intracellular; external side of plasma membrane

Molecular Function: growth factor activity; type 2 fibroblast growth factor receptor binding; receptor binding; fibroblast growth factor receptor binding; type 1 fibroblast growth factor receptor binding; chemoattractant activity

Biological Process: heart morphogenesis; heart development; multicellular organismal development; adrenocorticotropin hormone secreting cell differentiation; motor axon guidance; Wnt receptor signaling pathway through beta-catenin; mesodermal cell migration; BMP signaling pathway; positive chemotaxis; induction of an organ; male genitalia development; pallium development; negative regulation of neuron apoptosis; kidney development; regulation of odontogenesis of dentine-containing teeth; central nervous system neuron development; inner ear morphogenesis; fibroblast growth factor receptor signaling pathway; cell fate commitment; positive regulation of mitosis; neural plate morphogenesis; subpallium development; forebrain morphogenesis; dorsal/ventral axon guidance; patterning of blood vessels; forebrain neuron development; positive regulation of cell division; midbrain-hindbrain boundary development; response to oxidative stress; determination of left/right symmetry; negative regulation of apoptosis; gonad development; apoptosis; cell proliferation in forebrain; forebrain dorsal/ventral pattern formation; thyroid stimulating hormone secreting cell differentiation; embryonic hindlimb morphogenesis; embryonic development ending in birth or egg hatching; positive regulation of cell proliferation; thyroid gland development; heart looping; cell differentiation; otic vesicle formation; negative regulation of cardiac muscle development; pharyngeal system development; MAPKKK cascade; limb morphogenesis; positive regulation of organ growth; telencephalon development; generation of neurons; ureteric bud branching; embryonic heart tube development; blood vessel remodeling; lung development