Latexin Active Protein | LXN active protein

Latexin Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122500

• Purity: >95% as determined by SDS-PAGE
• Synonyms: Latexin; Latexin Protein; Mouse; Recombinant (His Tag); Mouse LXN/Latexin Protein (His Tag); latexin; LXN active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH8.0, 10% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Glu2-Glu222

• Species: Mouse
• Activity: Measured by its ability to inhibit carboxypeptidase-A1 cleavage of the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5'Dithiobis(2-nitrobenzoic acid)(DTNB). The IC50 value is <2.0nM.
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mouse LXN (NP_058033.2)(Glu 2-Glu 222) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for LXN active protein

Background: Latexin, also known as endogenous carboxypeptidase inhibitor, tissue carboxypeptidase inhibitor, TCI, ECI, and LXN, is a cytoplasm protein that belongs to the protease inhibitor I47 (latexin) family. It is highly expressed in the heart, prostate, ovary, kidney, pancreas, and colon. Latexin/LXN is the only known endogenous specific inhibitor of zinc-dependent metallocarboxypeptidases (MCPs) present in mammalians so far. Latexin is originally identified as a molecular marker for the regional specification of the neocortex in development in rats. The 222 amino acid latexin in the human shows different expression distribution with high levels in heart, prostate, ovary, kidney, pancreas, and colon, but only moderate or low levels in other tissues including the brain. Latexin is also expressed at high levels and is inducible in macrophages in concert with other protease inhibitors and potential protease targets, and thus is suggested to play a role in inflammation and innate immunity pathways. Despite the non-detectable sequence similarity with plant and parasite inhibitors, Latexin is related to a human putative tumor suppressor protein, TIG1. Also, Latexin is implicated in Alzheimer's disease.

References

Liu Q., et al.,(2000), Cloning, tissue expression pattern and genomic organization of latexin, a human homologue of rat carboxypeptidase A inhibitor. Mol. Biol. Rep. 27:241-246.Gauci S., et al., (2009), Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.nal. Chem. 81:4493-4501.Muzny D.M., et al.,(2006), The DNA sequence, annotation and analysis of human chromosome 3.Nature 440:1194-1198. Edwards, K.M. et al, 1999, J. Biol. Chem. 274:30468

NCBI and Uniprot Product Information

• NCBI GI #: 31980632
• NCBI GeneID: 17035
• NCBI Accession #: NP_058033.2
• NCBI GenBank Nucleotide #: NM_016753.4
• UniProt Accession #: P70202; Q3UDQ0; Q8CCS8
• Molecular Weight: 25,492 Da
• NCBI Official Full Name: latexin
• NCBI Official Synonym Full Names: latexin
• NCBI Official Symbol: Lxn
• NCBI Protein Information: latexin
• UniProt Protein Name: Latexin
• Protein Family: Latexin
• UniProt Gene Name: Lxn
• UniProt Synonym Gene Names: ECI; TCI
• UniProt Entry Name: LXN_MOUSE

Uniprot Description

LXN: Hardly reversible, non-competitive, and potent inhibitor of CPA1, CPA2 and CPA4. Belongs to the protease inhibitor I47 (latexin) family.

Protein type: Inhibitor

Molecular Function: metalloendopeptidase inhibitor activity

Biological Process: detection of temperature stimulus involved in sensory perception of pain