Galectin 7 Active Protein | LGALS7 active protein

Galectin 7 Protein, Human, Recombinant (GST Tag)

Cat. Num. AAA8121050

• Gene Names: LGALS7B; PI7; GAL7; Gal-7; HKL-14; LGALS7
• Purity: >92% as determined by SDS-PAGE
• Synonyms: Galectin 7; Galectin 7 Protein; Human; Recombinant (GST Tag); Human Galectin-7/LGALS7 Protein (GST Tag); GAL7 Protein; LGALS7A Protein; lectin; galactoside-binding; soluble; 7; LGALS7 active protein

• Host: E Coli
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ser2-Phe136

• Species: Human
• Activity: Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is typically 0.01-0.05ug/mL.
• Predicted N Terminal: Met
• Tag: N-GST
• Protein Construction: A DNA sequence encoding the human LGALS7 (P47929)(Ser 2-Phe 136) was fused with the GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for LGALS7 active protein

Background: LGALS7, also known as Galectin-7, is a member of the galectins family. The galectins are a family of beta-galactoside-binding proteins. There are at least 14 identified members of this family. Galectins share similarities in the CRD (the carbohydrate recognition domain). They are synthesized as cytosolic proteins. Though localized principally in the cytoplasm and lacking a classical signal peptide, galectins can also be stimulated to secretion by non-classical pathways or targeted to the nucleus. Galectins are implicated in modulating cell-cell and cell-matrix interactions. LGALS7 contains 1 galectin domain and is mainly expressed in stratified squamous epithelium. Galectin-7 could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. LGALS7 is a pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.

References

Villeneuve C, et al. (2011) Mitochondrial proteomic approach reveals galectin-7 as a novel BCL-2 binding protein in human cells. Mol Biol Cell. 22(7):999-1013.Rondanino C, et al. (2011) Galectin-7 modulates the length of the primary cilia and wound repair in polarized kidney epithelial cells. Am J Physiol Renal Physiol. 301(3):F622-33.Masuyer G, et al. (2012) Inhibition mechanism of human galectin-7 by a novel galactose-benzylphosphate inhibitor. FEBS J. 279(2):193-202.Magnaldo T, et al. (1995) Galectin-7, a human 14-kDa S-lectin, specifically expressed in keratinocytes and sensitive to retinoic acid. Dev Biol. 168(2):259-71.Madsen P, et al. (1995) Cloning, expression, and chromosome mapping of human galectin-7. J Biol Chem. 270(11):5823-29.

NCBI and Uniprot Product Information

• NCBI GI #: 109948279
• NCBI GeneID: 653499
• NCBI Accession #: NP_001035972.1
• NCBI GenBank Nucleotide #: NM_001042507.3
• UniProt Accession #: P47929; Q6IB87
• Molecular Weight: 15,075 Da
• NCBI Official Full Name: galectin-7
• NCBI Official Synonym Full Names: lectin, galactoside-binding, soluble, 7B
• NCBI Official Symbol: LGALS7B
• NCBI Official Synonym Symbols: PI7; GAL7; Gal-7; HKL-14; LGALS7
• NCBI Protein Information: galectin-7; galectin 7B; galectin-7B; p53-induced protein 1; keratinocyte lectin 14; p53-induced gene 1 protein; lectin galactoside-binding soluble 7
• UniProt Protein Name: Galectin-7
• Protein Family: Galectin
• UniProt Gene Name: LGALS7
• UniProt Synonym Gene Names: PIG1; Gal-7
• UniProt Entry Name: LEG7_HUMAN

NCBI Description

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridization studies indicate that this lectin is specifically expressed in keratinocytes and found mainly in stratified squamous epithelium. A duplicate copy of this gene (GeneID:3963) is found adjacent to, but on the opposite strand on chromosome 19. [provided by RefSeq, Jul 2008]

Uniprot Description

LGALS7B: Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.

Protein type: Cell adhesion; Apoptosis

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space; cytoplasm; nucleus

Molecular Function: carbohydrate binding

Biological Process: heterophilic cell adhesion; apoptosis