Stem Cell Factor Active Protein | k9SCF active protein

Recombinant Canine Stem Cell Factor

Cat. Num. AAA144769

• Purity: Greater than 97.0% as determined by HPLC and SDS-PAGE.
• Synonyms: Stem Cell Factor; Recombinant Canine Stem Cell Factor; SCF k9; Stem Cell Factor Canine Recombinant; Kit ligand Precursor; C-kit ligand; SCF; Mast cell growth factor; MGF; SF; KL-1; Kitl; DKFZp686F2250; k9SCF active protein

• Host: E Coli
• Purity/Purification: Greater than 97.0% as determined by HPLC and SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: KGICGKRVTD DVKDVTKLVA NLPKDYKIAL KYVPGMDVLP SHCWISVMVE QLSVSLTDLL DKFSNISEGL SNYSIIDKLV KIVDDLVECT EGYSFENVKK APKSPELRLF TPEEFFRIFN RSIDAFKDLE TVASKSSECV VSSTLSPDKD SRVSVTKPFM LPPVA
• Sequence Length: 37

• Solubility: It is recommended to reconstitute the lyophilized Stem Cell Factor in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: Fully biologically active when compared to standard. The ED50 as calculated by the dose-dependent stimulation of the proliferation of human TF-1 cells is less than 2.0ng/ml, corresponding to a specific activity of 5,000,000IU/mg.
• Preparation and Storage: Lyophilized SCF although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution SCF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for k9SCF active protein

Description: Stem Cell Factor Canine Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18.4kDa Dalton. The SCF is purified by proprietary chromatographic techniques.

Introduction: Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117(c-Kit). SCF is also known as "steel factor" or "c-kit ligand". SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases. SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).

Product Categories/Family for k9SCF active protein

Cytokines and Growth Factors; Growth Factors; Stem Cell Factor

NCBI and Uniprot Product Information

• NCBI GI #: 1246100
• Molecular Weight: 4,270 Da
• NCBI Official Full Name: stem cell factor, partial
• UniProt Protein Name: Stem cell factor
• UniProt Gene Name: stem cell factor: SCF
• UniProt Entry Name: Q13528_HUMAN

NCBI Description

This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]