interleukin 3 Active Protein | IL-3 active protein

Human interleukin 3

Cat. Num. AAA400020

• Gene Names: IL3; IL-3; MCGF
• Purity: Greater than 98.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Gel filtration Chromatoigraphy; (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
• Synonyms: interleukin 3; Human interleukin 3; Human interleukin 3 (IL-3); IL-3 active protein

• Host: E Coli
• Purity/Purification: Greater than 98.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Gel filtration Chromatoigraphy; (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
• Form/Format: Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Thr-Gln.
• Sequence Length: 143

• Solubility: It is recommended to reconstitute the lyophilized rHuIL-3 in sterile 18Momega-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Content: Protein quantitation was carried out by two independent methods:; 1. UV spectroscopy at 280 nm using the absorbency value of 0.84 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).; 2. Analysis by RP-HPLC, using a standard solution of IL-3 as a Reference Standard.
• Dimers and Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
• Endotoxin: Less than 0.1 ng/ug (IEU/ug) of rHuIL-3.
• Formulation: Lyophilized from a concentrated (1mg/ml) solution in water containing 0.3 mg/ml of NaHCO3.
• Biological Activity: rHuIL-3 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of TF-1 cells is less then 0.1 ng/ml, corresponding to a Specific Activity of 1 x 107 IU/mg.
• Preparation and Storage: Lyophilized rHuIL-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution rHuIL-3 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).; Please avoid freeze-thaw cycles.

Related Product Information for IL-3 active protein

Interleukin-3 (IL-3) is a cytokine produced by activated T-cells and mast cells that is active on a broad range of hematopoietic cells and in the nervous system and appears to be important in several chronic inflammatory diseases
Recombinant Human IL-3 is a single, non-glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton.

Product Categories/Family for IL-3 active protein

Hormones; Cytokines and Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 62078415
• NCBI GeneID: 481497
• NCBI Accession #: NP_001013857.1
• NCBI GenBank Nucleotide #: NM_001013835.1
• UniProt Accession #: Q9BDX4
• Molecular Weight: 16,499 Da
• NCBI Official Full Name: interleukin-3
• NCBI Official Symbol: IL3
• NCBI Official Synonym Symbols: IL-3; MCGF
• NCBI Protein Information: interleukin-3; P-cell-stimulating factor; hematopoietic growth factor; mast cell growth factor; multipotential colony-stimulating factor
• UniProt Protein Name: Interleukin-3
• UniProt Gene Name: IL3
• UniProt Synonym Gene Names: IL-3; MCGF
• UniProt Entry Name: IL3_CANFA

Uniprot Description

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.