Interleukin-16 Active Protein | IL 16 active protein

Recombinant Human Interleukin-16, (130 a.a.)

Cat. Num. AAA143194

• Gene Names: IL16; LCF; NIL16; PRIL16; prIL-16
• Purity: Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis SDS-PAGE.
• Synonyms: Interleukin-16; Recombinant Human Interleukin-16; (130 a.a.); IL 16 Human; Interleukin-16 Human Recombinant; IL16; LCF; Lymphocyte Chemoattractant Factor; prIL-16; IL-16; FLJ16806; FLJ42735; FLJ44234; HsT19289; IL 16; IL 16 active protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis SDS-PAGE.
• Form/Format: IL-16 was lyophilized at 1 mg/ml in 10mM NaP, pH-7.5.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Pro-Asp-Leu-Asn
• Sequence Length: 1331

• Solubility: It is recommended to reconstitute the lyophilized Interleukin-16 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The ED50 as determined by its ability to chemoattract human CD4+ T lymphocytes using a concentration range of 10-100ng/ml, corresponding to a Specific Activity of 10,000-100,000 units/mg.
• Preparation and Storage: Lyophilized Interleukin-16 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IL16 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for IL 16 active protein

Description: Interleukin-16 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 13.5 kDa. The IL-16 is purified by proprietary chromatographic techniques.

Introduction: IL-16 is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of IL-16 is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. IL-16 functions exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Two transcript variants encoding different isoforms have been found for this gene.IL-16 stimulates a migratory response in cd4+ lymphocytes, monocytes, and eosinophils. Also induces t-lymphocyte expression of interleukin 2 receptor, ligand for cd4.

Product Categories/Family for IL 16 active protein

Cytokines and Growth Factors; Cytokines; Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 289063395
• NCBI GeneID: 3603
• NCBI Accession #: NP_001165599.1
• NCBI GenBank Nucleotide #: NM_001172128.1
• UniProt Accession #: Q14005; Q16435; Q6VVE6; Q6ZMQ7; Q9UP18; A6NM20; A8MU65; B5TY35; B9EGR6; H3BVH5
• Molecular Weight: 59,345 Da
• NCBI Official Full Name: pro-interleukin-16 isoform 3
• NCBI Official Synonym Full Names: interleukin 16
• NCBI Official Symbol: IL16
• NCBI Official Synonym Symbols: LCF; NIL16; PRIL16; prIL-16
• NCBI Protein Information: pro-interleukin-16; lymphocyte chemoattractant factor; neuronal interleukin 16; prointerleukin 16
• UniProt Protein Name: Pro-interleukin-16
• UniProt Gene Name: IL16
• UniProt Synonym Gene Names: IL-16; LCF
• UniProt Entry Name: IL16_HUMAN

NCBI Description

The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

Uniprot Description

IL16: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Cytokine; Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: extracellular space; cytoplasm; plasma membrane; nucleus

Molecular Function: cytokine activity

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; viral reproduction; leukocyte chemotaxis; immune response; induction of positive chemotaxis