Insulin Like Growth Factor Binding Protein-5 Active Protein | IGFBP5 active protein

Recombinant Human Insulin Like Growth Factor Binding Protein-5

Cat. Num. AAA142340

• Gene Names: IGFBP5; IBP5
• Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Insulin Like Growth Factor Binding Protein-5; Recombinant Human Insulin Like Growth Factor Binding Protein-5; IGFBP 5 Human; Insulin-Like Growth Factor Binding Protein-5 Human Recombinant; IGFBP-5; IBP-5; IGF-binding protein 5; IGFBP5 active protein

• Host: E Coli
• Purity/Purification: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: IBP-5 was lyophilized from a concentrated (1mg/ml) solution containing 10mM sodium Citrate pH-3; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: LGSFVHCEPC DEKALSMCPP SPLGCELVKE PGCGCCMTCA LAEGQSCGVY TERCAQGLRC LPRQDEEKPL HALLHGRGVC LNEKSYREQV KIERDSREHE EPTTSEMAEE TYSPKIFRPK HTRISELKAE AVKKDRRKKL TQSKFVGGAE NTAHPRIISA PEMRQESEQG PCRRHMEASL QELKASPRMV PRAVYLPNCD RKGFYKRKQC KPSRGRKRGI CWCVDKYGMK LPGMEYVDGD FQCHTFDSSN VE
• Sequence Length: 272

• Solubility: It is recommended to reconstitute the lyophilized Insulin-Like Growth Factor Binding Protein-5 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The ED50, calculated by its ability to inhibit IGF-II induced proliferation of MCF-7. The expected ED50 for this effect is < 0.4 ?g/ml, corresponding to a specific activity of > 2500 IU/mg in the presence of 15 ng/ml of rHuIGF-II.
• Preparation and Storage: Lyophilized IBP5 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IGFBP 5 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for IGFBP5 active protein

Description: IGFBP5 Human Recombinant produced in E Coli is a single, non-glycosylated, homodimeric polypeptide chain containing 252 amino acids and having a molecular mass of 28.6 kDa. IGFBP5 is purified by proprietary chromatographic techniques.

Introduction: IGFBP5 is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Product Categories/Family for IGFBP5 active protein

Cytokines and Growth Factors; Growth Factors; IGFBP

NCBI and Uniprot Product Information

• NCBI GI #: 10834982
• NCBI GeneID: 3488
• NCBI Accession #: NP_000590.1
• NCBI GenBank Nucleotide #: NM_000599.3
• UniProt Accession #: P24593; Q5U0A3
• Molecular Weight: 30,570 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 5
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 5
• NCBI Official Symbol: IGFBP5
• NCBI Official Synonym Symbols: IBP5
• NCBI Protein Information: insulin-like growth factor-binding protein 5; IBP-5; IGF-binding protein 5; IGFBP-5
• UniProt Protein Name: Insulin-like growth factor-binding protein 5
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: IGFBP5
• UniProt Synonym Gene Names: IBP5; IBP-5; IGF-binding protein 5; IGFBP-5
• UniProt Entry Name: IBP5_HUMAN

Uniprot Description

IGFBP5: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Protein type: Secreted, signal peptide; Secreted; Cell cycle regulation

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: insulin-like growth factor binding protein complex; extracellular space; extracellular region

Molecular Function: protein binding; insulin-like growth factor I binding; fibronectin binding; insulin-like growth factor II binding

Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; mammary gland involution; hair follicle morphogenesis; negative regulation of smooth muscle cell proliferation; negative regulation of insulin-like growth factor receptor signaling pathway; glucose metabolic process; negative regulation of smooth muscle cell migration; signal transduction; glucose homeostasis; osteoblast differentiation; positive regulation of protein kinase B signaling cascade; cellular protein metabolic process; negative regulation of translation; striated muscle cell differentiation; negative regulation of osteoblast differentiation; regulation of cell growth; skeletal muscle growth; negative regulation of cell migration