TFF-3 active protein

Human TFF-3

Cat. Num. AAA691923

• Gene Names: TFF3; ITF; P1B; TFI
• Reactivity: Human
• Purity: > 98% by SDS-PAGE analyses
• Synonyms: TFF-3; Human TFF-3; Recombinant Human Trefoil Factor 3; TFF-3 active protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: > 98% by SDS-PAGE analyses
• Form/Format: Lyophilized
• Sequence: EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVP WCFK PLQEAECTF
• Sequence Length: 94

• Endotoxin Level: < 0.1 ng per g of TFF-3
• Biological Activity: Determined by its ability to chemoattract human MCF-7 cells using a concentration range of 1.0-10.0 ug/ml.

Related Product Information for TFF-3 active protein

The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are secreted in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF-3 is expressed by goblet cells and in the uterus, and has also been shown to express in certain cancers, including colorectal, hepatocellular, and in biliary tumors. TFF3 may be useful as a molecular marker for certain types of cancer, but its role, if any, in tumorigenesis is unknown. TFF3 also promotes airway epithelial cell migration and differentiation. Recombinant human TFF3 is a 13.2 kDa homodimeric protein consisting of two 59 amino acid chains, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.

Product Categories/Family for TFF-3 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 281485608
• NCBI GeneID: 7033
• NCBI Accession #: NP_003217.3
• NCBI GenBank Nucleotide #: NM_003226.3
• UniProt Accession #: Q07654; Q96NX0; Q9UDA5; E9PBB5
• Molecular Weight: 13.2 kDa
• NCBI Official Full Name: trefoil factor 3
• NCBI Official Synonym Full Names: trefoil factor 3 (intestinal)
• NCBI Official Symbol: TFF3
• NCBI Official Synonym Symbols: ITF; P1B; TFI
• NCBI Protein Information: trefoil factor 3; polypeptide P1.B
• UniProt Protein Name: Trefoil factor 3
• UniProt Gene Name: TFF3
• UniProt Synonym Gene Names: ITF; TFI; hITF; hP1.B
• UniProt Entry Name: TFF3_HUMAN

NCBI Description

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen). Ref.12

Subunit structure: Monomer. Homodimer; disulfide-linked. Ref.14

Subcellular location: Secreted › extracellular space › extracellular matrix › apical lamina Ref.8.

Tissue specificity: Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus. Ref.1 Ref.8

Sequence similarities: Contains 1 P-type (trefoil) domain.

Sequence caution: The sequence AAH17859.1 differs from that shown. Reason: Erroneous initiation. The sequence AAL28111.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA95531.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB13731.1 differs from that shown. Reason: Erroneous initiation.