PAI-1 active protein

Human PAI-1

Cat. Num. AAA691990

• Gene Names: SERPINE1; PAI; PAI1; PAI-1; PLANH1
• Reactivity: Human
• Purity: > 95% by SDS-PAGE & HPLC analyses
• Synonyms: PAI-1; Human PAI-1; Plasminogen Activator Inhibitor-1; Serpin E1; PAI-1 active protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: > 95% by SDS-PAGE & HPLC analyses
• Form/Format: Lyophilized (50mM Sodium Acetate, pH 5.5 + 100mM NaCI)
• Sequence: VHHPPSYVAH LASDFGVRVF QQVAQASKDR NVVFSPYGVA SVLAML QLTT GGETQQQIQA AMGFKIDDKG MAPALRHLYK ELMGPWNKDE I STTDAIFVQ RDLKLVQGFM PHFFRLFRST VKQVDFSEVE RARFIIN DWV KTHTKGMISN LLGKGAVDQL TRLVLVNALY FNGQWKTPFP DS STHRRLFH KSDGSTVSVP MMAQTNKFNY TEFTTPDGHY YDILELPY HG DTLSMFIAAP YEKEVPLSAL TNILSAQLIS HWKGNMTRLP RLL VLPKFSL ETEVDLRKPL ENLGMTDMFR QFQADFTSLS DQEPLHVAQ A LQKVKIEVNE SGTVASSSTA VIVSARMAPE EIIMDRPFLF VVRH NPTGTV LFMGQVMEP
• Sequence Length: 402

• Endotoxin Level: < 0.1 ng/ug of protein (<1EU/ug).Biological Activity
• Length: 379
• Stability: The lyophilized protein is stable at room temperature for 1 month and at 4°C for 3 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/m. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Related Product Information for PAI-1 active protein

Plasminogen Activator Inhibitor-1 (PAI-1, Serpin E1) is a member of the serpin family of serine protease inhibitors, and is the primary inhibitor of urokinase and tissue plasminogen activator (tPA). PAI-1 is expressed predominantly in adipose, liver and vascular tissues, and is also produced by certain tumor cells. Elevated levels of PAI-1 are associated with obesity, diabetes and cardiovascular disease, and increased production of PAI-1 is induced by various obesity related factors such as TNFalpha, glucose, insulin, and very-low-density lipoprotein. The obesity related elevation of PAI-1 levels along with the consequential deficiency in plasminogen activators can lead directly to increased risk of thrombosis and other coronary diseases. Accordingly, PAI-1 has been implicated as an important molecular link between obesity and coronary disease. PAI-1 can also specifically bind vitronectin (VTN) to form a stable active complex with an increased circulatory half life relative to free PAI-1. Recombinant human PAI-1 is a 42.7 kDa protein containing 379 amino acid residues.

Product Categories/Family for PAI-1 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 10835159
• NCBI GeneID: 5054
• NCBI Accession #: NP_000593.1
• NCBI GenBank Nucleotide #: NM_000602.4
• UniProt Accession #: P05121; B7Z4S0; F8WD53
• Molecular Weight: 42.7 kDa
• NCBI Official Full Name: plasminogen activator inhibitor 1
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
• NCBI Official Symbol: SERPINE1
• NCBI Official Synonym Symbols: PAI; PAI1; PAI-1; PLANH1
• NCBI Protein Information: plasminogen activator inhibitor 1; serpin E1; endothelial plasminogen activator inhibitor; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
• UniProt Protein Name: Plasminogen activator inhibitor 1
• UniProt Gene Name: SERPINE1
• UniProt Synonym Gene Names: PAI1; PLANH1; PAI; PAI-1
• UniProt Entry Name: PAI1_HUMAN

NCBI Description

This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

Function: Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis. Ref.18

Subunit structure: Forms protease inhibiting heterodimer with TMPRSS7. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Ref.15 Ref.17

Subcellular location: Secreted.

Tissue specificity: Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.

Post-translational modification: Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-|-Met-370 bond.

Involvement in disease: Plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]: A hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.16High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.

Sequence similarities: Belongs to the serpin family.