MIA (Melanoma inhibitory activity) Active Protein | MIA active protein
Human MIA (Melanoma inhibitory activity)
Gene Names
MIA; CD-RAP
Reactivity
Human
Purity
> 98% by SDS-PAGE and HPLC analysis
Synonyms
MIA (Melanoma inhibitory activity); Human MIA (Melanoma inhibitory activity); Recombinant Human MIA; MIA active protein
Host
E Coli
Reactivity
Human
Purity/Purification
> 98% by SDS-PAGE and HPLC analysis
Form/Format
Lyophilized; 15mM Sodium Phosphate, pH 7.2 + 75mM NaC1
Sequence
MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQV VYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT L KPGKVDVKT DKWDFYCQ
Sequence Length
131
Endotoxin Level
< 0.1 ng/ug of protein (<1EU/ug)
Reconstitution
Centrifuge vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Biological Activity
The ED50 was determined by the dose-dependant proliferation of the human A375 melanoma cell line. The expected ED50 for this effect is 4-6 ug/ml.
Length (aa)
108
Preparation and Storage
The lyophilized protein is stable at 4°C. Reconstituted working aliquots are stable at 2°C to 8°C.
Related Product Information for MIA active protein
MIA is the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family; MIA, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. MIA is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of MIA may represent a clinically useful marker for identification of melanoma metastasis as well as a potential marker for rheumatoid arthritis. Recombinant human MIA is a 12.2 kDa globular protein containing 108 amino acid residues including two intramolecular disulfide bonds..
Product Categories/Family for MIA active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12.2 kDa
NCBI Official Full Name
melanoma-derived growth regulatory protein
NCBI Official Synonym Full Names
melanoma inhibitory activity
NCBI Official Symbol
MIA
NCBI Official Synonym Symbols
CD-RAP
NCBI Protein Information
melanoma-derived growth regulatory protein
UniProt Protein Name
Melanoma-derived growth regulatory protein
Protein Family
UniProt Gene Name
MIA
UniProt Entry Name
MIA_HUMAN
Uniprot Description
Function: Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
Subunit structure: Interacts with FASLG. Interacts with TMIGD2. Ref.9 Ref.10
Subcellular location: Secreted.
Tissue specificity: All malignant melanoma cell lines tested and infrequently in glioma cell lines.
Post-translational modification: May possess two intramolecular disulfide bonds.
Sequence similarities: Belongs to the MIA/OTOR family.Contains 1 SH3 domain.
Research Articles on MIA
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Product Notes
The MIA mia (Catalog #AAA691728) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Human MIA (Melanoma inhibitory activity) reacts with Human and may cross-react with other species as described in the data sheet. The amino acid sequence is listed below: MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQV VYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT L KPGKVDVKT DKWDFYCQ. It is sometimes possible for the material contained within the vial of "MIA (Melanoma inhibitory activity), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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