IGF-BP3 active protein

Human IGF-BP3

Cat. Num. AAA692057

• Gene Names: IGFBP3; IBP3; BP-53
• Reactivity: Human
• Purity: > 98% by SDS-PAGE and HPLC analysis
• Synonyms: IGF-BP3; Human IGF-BP3; Recombinant Human IGF-BP3; IGF-BP3 active protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: > 98% by SDS-PAGE and HPLC analysis
• Form/Format: Lyophilized
• Sequence: GASSGGLGPV VRCEPCDARA LAQCAPPPAV CAELVREPGC GCCLTC ALSE GQPCGIYTER CGSGLRCQPS PDEARPLQAL LDGRGLCVNA S AVSRLRAYL LPAPPAPGNA SESEEDRSAG EVESPSVSST HRVSDPK FHP LHSKIIIIKK GHAKDSQRYK VDYESQSTDT QNFSSESKRE TE YGPCRREM EDTLNHLKFL NVLSPRGVHI PNCDKKGFYK KKQCRPSK GR KRGFCWCVDK YGQPLPGYTT KGKEDVHCYS MQSK
• Sequence Length: 291

• Endotoxin Level: < 0.1 ng per ug of IGF-BP3
• Biological Activity: The ED50 was determined by its ability to inhibit IGF-II induced proliferation of MCF-7. The expected ED50 for this effect is <= 0.2 ug/ml in presence of 15 ng/ml of human IGF-II.

Related Product Information for IGF-BP3 active protein

Human IGF-BP3, also known as growth-hormone-dependant binding protein, is 28.9 kDa cysteine-rich secreted protein. It is the major IGF binding protein present in the plasma of human and animals and it is also found in alpha-granules of platelets. In addition to its ability to modulate the activity of IGF-I and IGF-2, IGF-BP3 exerts inhibitory effects on follicle stimulating hormone (FSH) activity. Decreased plasma levels of IGF-BP3 often results in dwarfism, whereas elevated levels of IGF-BP3 may lead to acromegaly. The expression of IGF-BP3 in fibroblasts is stimulated by mitogenic growth factors such as Bombesin, Vasopressin, PDGF and EGF. Recombinant human IGF-BP3 is a 28.8 kDa protein consisting of 264 amino acid residues.

Product Categories/Family for IGF-BP3 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 62243068
• NCBI GeneID: 3486
• NCBI Accession #: NP_000589.2
• NCBI GenBank Nucleotide #: NM_000598.4
• UniProt Accession #: P17936; Q2V509; Q6P1M6; Q9UCL4; A4D2F5; D3DVM0
• Molecular Weight: 28.8 kDa
• NCBI Official Full Name: insulin-like growth factor-binding protein 3 isoform b
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 3
• NCBI Official Symbol: IGFBP3
• NCBI Official Synonym Symbols: IBP3; BP-53
• NCBI Protein Information: insulin-like growth factor-binding protein 3; IBP-3; IGFBP-3; binding protein 29; binding protein 53; IGF-binding protein 3; growth hormone-dependent binding protein; acid stable subunit of the 140 K IGF complex
• UniProt Protein Name: Insulin-like growth factor-binding protein 3
• UniProt Gene Name: IGFBP3
• UniProt Synonym Gene Names: IBP3; IBP-3; IGF-binding protein 3; IGFBP-3
• UniProt Entry Name: IBP3_HUMAN

NCBI Description

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Ref.17

Subunit structure: Interacts with XLKD1

By similarity. Binds IGF2 more than IGF1. Forms a ternary complex of about 140 to 150 kDa with IGF1 or IGF2 and a 85 kDa glycoprotein (ALS). Interacts with HN. Interacts with TMEM219. Ref.10 Ref.12 Ref.17

Subcellular location: Secreted Ref.17.

Tissue specificity: Expressed by most tissues. Present in plasma.

Developmental stage: IGFBP3 levels are higher during extrauterine life and peak during puberty.

Induction: Up-regulated in the presence of IGF1, insulin and other growth-stimulating factors such as growth hormone, EGF and phorbol esters.

Domain: The thyroglobulin type-1 domain mediates interaction with HN.

Post-translational modification: Phosphorylation sites are present in the extracellular medium.

Sequence similarities: Contains 1 IGFBP N-terminal domain.Contains 1 thyroglobulin type-1 domain.