Apo-SAA active protein

Human Apo-SAA

Cat. Num. AAA691935

• Gene Names: SAA1; SAA; PIG4; SAA2; TP53I4
• Reactivity: Human
• Purity: > 98% by SDS-PAGE and HPLC analyses
• Synonyms: Apo-SAA; Human Apo-SAA; Recombinant Human Apo-SAA; Apo-SAA active protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: > 98% by SDS-PAGE and HPLC analyses
• Form/Format: Lyophilized (5mMTris, pH7.8)
• Sequence: MRSFFSFLGE AFDGARDMWR AYSDMREANY IGSDKYFHAR GNYDAA KRGP GGVWAAEAIS NARENIQRFF GRGAEDSLAD QAANEWGRSG K DPNHFRPAG LPEKY
• Sequence Length: 122

• Endotoxin Level: < 0.1 ng /ug of protein (<1EU/ug).
• Stability: The lyophilized protein is stable at room temperature for 1 month and at 4°C for 6 months. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/m. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containinga carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C
• Length: 104
• Biological Activity: Tested by its ability to down-regulate lipid biosynthesis in aortic smooth muscle cells. The effective concentration was found to be 4 uM.

Related Product Information for Apo-SAA active protein

Human Apo-SAA is a 104 amino acid polypeptide that circulates primarily in association with high-density lipoproteins (HDL). The level of Apo-SAA, normally 1-5 ug/ml in plasma, increases 500-1000 fold within 24 hours of an inflammatory stimulus and, under these conditions, is the most abundant HDL Apolipoprotein. Thehuman SAA gene codes for a 122 amino acid polypeptide, which contains an 18 amino acid N-terminal signal sequence. Recombinant Apo-SAA is a consensus SAA molecule corresponding to human Apo-SAA1alpha except for the presence of an N-terminal methionine andsubstitution of asparagine for aspartic acid at position 60 and arginine for histidine at position 71(the last two substituted residues are present in apo-SAA2beta).The calculated molecular weight of recombinant human Apo-SAAis 11.7 kDa.

Product Categories/Family for Apo-SAA active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 40316912
• NCBI GeneID: 6288
• NCBI Accession #: NP_000322.2
• NCBI GenBank Nucleotide #: NM_000331.4
• UniProt Accession #: P0DJI8; P02735; P02736; P02737; Q16730; Q16834; Q16835; Q16879; Q3KRB3; Q6FG67; Q96QN0; Q9UCK9
• Molecular Weight: ~11.7 kDa
• NCBI Official Full Name: serum amyloid A-1 protein preproprotein
• NCBI Official Synonym Full Names: serum amyloid A1
• NCBI Official Symbol: SAA1
• NCBI Official Synonym Symbols: SAA; PIG4; SAA2; TP53I4
• NCBI Protein Information: serum amyloid A-1 protein; serum amyloid A protein; tumor protein p53 inducible protein 4
• UniProt Protein Name: Serum amyloid A-1 protein
• UniProt Gene Name: SAA1
• UniProt Synonym Gene Names: SAA
• UniProt Entry Name: SAA1_HUMAN

NCBI Description

This gene encodes a member of the serum amyloid A family of apolipoproteins. The encoded protein is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors. Alternate splicing results in multiple transcript variants that encode the same protein. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Jun 2012]

Uniprot Description

Function: Major acute phase reactant. Apolipoprotein of the HDL complex.

Subcellular location: Secreted.

Tissue specificity: Expressed by the liver; secreted in plasma. Ref.18

Induction: Upon cytokine stimulation.

Post-translational modification: This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.

Polymorphism: At least 5 different SAA1 alleles have been described: SAA1.1 (SAA1alpha), SAA1.2 (SAA1beta), SAA1.3 (SAA1gamma), SAA1.4 (SAA1delta), SAA1.5 (also named SAA1beta but which differs from SAA1.2). We use here the revised nomenclature described in Ref.21. The sequence shown is that of SAA1.1.

Involvement in disease: Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA1 protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Ref.14Elevated serum SAA1 protein levels may be associated with lung cancer. Ref.14

Sequence similarities: Belongs to the SAA family.

Mass spectrometry: Molecular mass is 11702±14 Da from positions 19 - 122. Determined by MALDI. Ref.18Molecular mass is 11682.7 Da from positions 19 - 122. Determined by MALDI. Ref.22Molecular mass is 11526.5 Da from positions 20 - 122. Determined by MALDI. Ref.22Molecular mass is 11439.6 Da from positions 21 - 122. Determined by MALDI. Ref.22Molecular mass is 11363.6 Da from positions 20 - 121. Determined by MALDI. Ref.22Molecular mass is 11235.6 Da from positions 20 - 120. Determined by MALDI. Ref.22Molecular mass is 10872.6 Da from positions 22 - 119. Determined by MALDI. Ref.22Molecular mass is 8337.5±0.8 Da from positions 19 - 94. Determined by ESI. With variants Ala-70, Val-75, Asn-78 and 86-Leu-Thr-87. Ref.14Molecular mass is 8390.9±0.2 Da from positions 19 - 94. Determined by ESI. With variant Ala-70. Ref.14