Neuregulin-4 Active Protein | NRG4 active protein
Human Neuregulin-4 Recombinant
Purity
>96% as determined by SDS-PAGE and HPLC
Synonyms
Neuregulin-4; Human Neuregulin-4 Recombinant; egf; neuregulin; nrg4; nrg-4; heregulin; hrg; NRG4 active protein
Host
E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
Purity/Purification
>96% as determined by SDS-PAGE and HPLC
Form/Format
Recombinant Neuregulin-4 was lyophilized from a 0.2 um filtered 20mM PB, pH 6.0.
Host Note
Optimized DNA sequence encoding Human Neuregulin-4 EGF domain was expressed in Escherichia Coli.
Biological Activity:
The ED(50) was determined by the phosphorylation of ErbB2 and ErbB4 receptors in CHO cells, and was found to be <3ng.ml, corresponding to a specific activity of 3x 10^5 Units/mg.
Presentation
Recombinant Neuregulin-4 was lyophilized from a 0.2 f.lm filtered 20mM PB, pH 6.0.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Related Product Information for NRG4 active protein
Neuregulins (NDF, heregulin, GGF ARIA, or SMDF) are EGF- like growth and differentiation factors that signal through tyrosine kinase receptors of the ErbB family.The ErbB2 and ErbB4 receptors cooperate in transmission of neuregulin-1 signals in the heart, whereas ErbB2 and ErbB3 cooperate in neural crest cells.
Product Categories/Family for NRG4 active protein
References
• expression of neuregulin 4 splice variants in normal human tissues and prostate cancer and their effects on cell motility .Endocr. Relat. Cancer, Dec 2010; 18: 39 - 49.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Recombinant human Neuregulin-4 EGF domain is a disulfide-linked monomeric protein consisting of 62 amino acid residue subunits. and migrates as
an approximately 7 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
NCBI Official Full Name
pro-neuregulin-4, membrane-bound isoform
UniProt Protein Name
Pro-neuregulin-4, membrane-bound isoform
UniProt Gene Name
NRG4
UniProt Synonym Gene Names
Pro-NRG4; NRG-4
UniProt Entry Name
NRG4_HUMAN
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Product Notes
The NRG4 nrg4 (Catalog #AAA14428) is an Active Protein produced from E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Neuregulin-4, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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