Sequence
Paraoxonase 2 (PoN2) Active Protein | PoN2 active protein
Active Paraoxonase 2 (PoN2)
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Sequence
Sequence
Activity Data
(Paraoxonase 2 which is a member of the paraoxonase family also known as arylesterase 2. PON2 is exclusively intracellularly found, wherein it functions as an anti-oxidative protein by reducing intracellular and local oxidative stress. This protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Besides, ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) has been identified as an interactor of PON2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PON2 and recombinant human ATP6AP2. Briefly, PON2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ATP6AP2-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PON2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of PON2 and ATP6AP2 was shown in Figure 1, and this effect was in a dose dependent manner.)
Activity Data
(Paraoxonase 2 which is a member of the paraoxonase family also known as arylesterase 2. PON2 is exclusively intracellularly found, wherein it functions as an anti-oxidative protein by reducing intracellular and local oxidative stress. This protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Besides, ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) has been identified as an interactor of PON2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PON2 and recombinant human ATP6AP2. Briefly, PON2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ATP6AP2-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PON2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of PON2 and ATP6AP2 was shown in Figure 1, and this effect was in a dose dependent manner.)
SDS-PAGE
(Sample: Active recombinant PoN2, Human)
SDS-PAGE
(Sample: Active recombinant PoN2, Human)
Western Blot (WB)
(Sample: Recombinant PoN2, Human;Antibody: Rabbit Anti-Human PoN2 Ab (MBS2027275))
Western Blot (WB)
(Sample: Recombinant PoN2, Human;Antibody: Rabbit Anti-Human PoN2 Ab (MBS2027275))
Similar Products
Product Notes
The PoN2 (Catalog #AAA2090458) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The Active Paraoxonase 2 (PoN2) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Paraoxonase 2 (PoN2) can be used in a range of immunoassay formats including, but not limited to, Cell culture; Activity Assays. (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the PoN2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Paraoxonase 2 (PoN2), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
