PNGase F enzyme
PNGase F
Purity
>95% as determined by SDS-PAGE quantitative densitometry by Coomassie Blue Staining
Synonyms
PNGase F; PNGase F enzyme
Host
E Coli
Purity/Purification
>95% as determined by SDS-PAGE quantitative densitometry by Coomassie Blue Staining
Form/Format
Liquid. 20 mM Tris-HCl pH7.5, 50 mM NaCl, 5 mM EDTA, 50%Glycerol
Concentration
1,800,000 U/mg (varies by lot)
Unit Definition
One unit of PNGase F will catalyze more than 95% of the deglycosylation of 10ug of denatured Ribonuclease B (RNase B) in 10 ul reaction system in one hour at 37 degree C
Digestion Condition
At 37 degree C, the enzyme digestion reaction 1h, every 1ug enzyme can digest 10ug substrate.
Restriction Site
PNGase F works by cleaving between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins and glycopeptides. This results in a deaminated protein or peptide and a free glycan.
Preparation and Storage
Store at -20 degree C for ~12 months from the date of receipt
Product Categories/Family for PNGase F enzyme
Similar Products
Product Notes
The PNGase F (Catalog #AAA157417) is an Enzyme produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PNGase F, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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