BGrowth Hormone Active Protein | bGH active protein
Recombinant Bovine Growth Hormone
Gene Names
GH1; GH
Purity
Greater than 98.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Synonyms
BGrowth Hormone; Recombinant Bovine Growth Hormone; GH Bovine; Growth Hormone Bovine Recombinant; BGH; BST; rBGH; rBST; Bovine Somatotropin; Bovine GH; Growth hormone; GH1; bGH active protein
Host
E Coli
Purity/Purification
Greater than 98.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Form/Format
The Bovine Growth Hormone protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3 adjusted to pH 8-9.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sterile Filtered White lyophilized (freeze-dried) powder.
Concentration
1mg/ml (varies by lot)
Sequence Length
217
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.63 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of GH as a Reference Standard.
1. UV spectroscopy at 280 nm using the absorbency value of 0.63 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of GH as a Reference Standard.
Solubility
It is recommended to reconstitute the lyophilized Bovine Growth Hormone in 0.4% NaHCO3 or sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content
Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.68 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GH as a Reference Standard.
Biological Activity
The activity as determined by the PDFP13B9 cells stably transfected with rabbit GH receptors. Bovine GH is also capable of forming a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD).
Amino Acid Sequence
1 - afpamslsgl fanavlraqh lhqlaadtfk efertyipeg qrysiqntqv
51 - afcfsetmpa ptgkneaqqk sdlellrisl lliqswlgpl qflsrvftns
101 - lvfgtsdrvy eklkdleegi lalmreledg tprrgqilkq tydkfdtnmr
151 - sddallknyg llscfrkdlh ktetylrvmk crrfgeasca f
51 - afcfsetmpa ptgkneaqqk sdlellrisl lliqswlgpl qflsrvftns
101 - lvfgtsdrvy eklkdleegi lalmreledg tprrgqilkq tydkfdtnmr
151 - sddallknyg llscfrkdlh ktetylrvmk crrfgeasca f
Preparation and Storage
Lyophilized Bovine Growth Hormone although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution GH Bovine should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Related Product Information for bGH active protein
Growth Hormone Bovine Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 191 amino acids and having a molecular mass of 21.8 kDa. The GH Bovine Recombinant is purified by proprietary chromatographic techniques.
Product Categories/Family for bGH active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
somatotropin
NCBI Official Symbol
GH1
NCBI Official Synonym Symbols
GH
NCBI Protein Information
somatotropin
UniProt Protein Name
Somatotropin
UniProt Gene Name
GH1
UniProt Synonym Gene Names
GH
UniProt Entry Name
SOMA_BOVIN
Uniprot Description
Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
Research Articles on bGH
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Product Notes
The bGH gh1 (Catalog #AAA143497) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "BGrowth Hormone, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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