Aldose Reductase Active Protein | AKR1B1 active protein
Recombinant Mouse Aldose Reductase
Gene Names
Akr1b3; AR; ALR2; Ahr1; Ahr-1; Aldr1; Akr1b1; Aldor1
Purity
Greater than 95% as determined by SDS-PAGE.
Synonyms
Aldose Reductase; Recombinant Mouse Aldose Reductase; AKR1B1 Mouse; Aldose Reductase Mouse Recombinant; Aldose reductase; AKR1B1; AR; Aldehyde reductase; Akr1b3; Aldor1; Aldr1; Akr1b1; Ahr-1; Ahr1; ALR2; AKR1B1 active protein
Host
E Coli
Purity/Purification
Greater than 95% as determined by SDS-PAGE.
Form/Format
AKR1B1 protein solution (1mg/ml) containing Phosphate buffered saline (pH7.4), and 10% glycerol.
Sterile Filtered colorless solution.
Sterile Filtered colorless solution.
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMASHLEL NNGTKMPTLG LGTWKSPPGQ VTEAVKVAID LGYRHIDCAQ VYQNEKEVGV ALQEKLKEQV VKRQDLFIVS KLWCTFHDKS MVKGAFQKTL SDLQLDYLDL YLIHWPTGFK PGPDYFPLDA SGNVIPSDTD FVDTWTAMEQ LVDEGLVKTI GVSNFNPLQI ERILNKPGLK YKPAVNQIEC HPYLTQEKLI EYCHSKGIVV TAYSPLGSPD RPWAKPEDPS LLEDPRIKAI AAKYNKTTAQ VLIRFPIQRN LVVIPKSVTP VRIAENLKVF DFEVSSEDMA TLLSYNRNWR VCALMSCAKH KDYPFHAEV.
Sequence Length
316
Biological Activity
Specific activity is > 500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0 pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37C.
Related Product Information for AKR1B1 active protein
Introduction: AKR1B1 is part of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. AKR1B1 catalyzes the reduction several aldehydes, including the aldehyde form of glucose, and thus involved in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. AKR1B1 catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Transgenic mice over expressing human aldose reductase show that AKR1B1 is a key player in ischemic injury and impairment of functional and metabolic recovery after ischemia. Aldose Reductase is an obligatory mediator of TNF-alpha signaling leading to an increase in the expression of adhesion molecules and increased binding of monocytes to the endothelium. AKR1B1 is a critical regulator of TNF-alpha-induced apoptotic signaling in endothelial cells.
Description: AKR1B1 Mouse Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 339 amino acids (1-316a.a.) and having a molecular mass of 38.1kDa.AKR1B1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Description: AKR1B1 Mouse Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 339 amino acids (1-316a.a.) and having a molecular mass of 38.1kDa.AKR1B1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Categories/Family for AKR1B1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,732 Da
NCBI Official Full Name
aldose reductase
NCBI Official Synonym Full Names
aldo-keto reductase family 1, member B3 (aldose reductase)
NCBI Official Symbol
Akr1b3
NCBI Official Synonym Symbols
AR; ALR2; Ahr1; Ahr-1; Aldr1; Akr1b1; Aldor1
NCBI Protein Information
aldose reductase
UniProt Protein Name
Aldose reductase
Protein Family
UniProt Gene Name
Akr1b1
UniProt Synonym Gene Names
Akr1b3; Aldor1; Aldr1; AR
UniProt Entry Name
ALDR_MOUSE
Uniprot Description
AKR1B1: Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. Belongs to the aldo/keto reductase family.
Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - pentose and glucuronate interconversions; Carbohydrate Metabolism - pyruvate; EC 1.1.1.21; Lipid Metabolism - glycerolipid; Oxidoreductase
Cellular Component: cytoplasm; cytosol; extracellular space; mast cell granule; nucleoplasm; perinuclear region of cytoplasm
Molecular Function: aldehyde reductase activity; glyceraldehyde oxidoreductase activity
Biological Process: monosaccharide metabolic process; naphthalene metabolic process; negative regulation of apoptosis; norepinephrine metabolic process; positive regulation of JAK-STAT cascade; positive regulation of smooth muscle cell proliferation; renal water homeostasis; response to organic substance; response to water deprivation; sorbitol biosynthetic process; stress-activated protein kinase signaling pathway; tissue homeostasis
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Product Notes
The AKR1B1 akr1b1 (Catalog #AAA140704) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MGSSHHH HHH SSGLVPRGSH MGSMAS HLEL NNGTKMPTLG LGTWKSPPGQ VTEAVKVAID LGYRHIDCAQ VYQNEKEVGV ALQEKLKEQV VKRQDLFIVS KLWCTFHDKS MVKGAFQKTL SDLQLDYLDL YLIHWPTGFK PGPDYFPLDA SGNVIPSDTD FVDTWTAMEQ LVDEGLVKTI GVSNFNPLQI ERILNKPGLK YKPAVNQIEC HPYLTQEKLI EYCHSKGIVV TAYSPLGSPD RPWAKPEDPS LLEDPRIKAI AAKYNKTTAQ VLIRFPIQRN LVVIPKSVTP VRIAENLKVF DFEVSSEDMA TLLSYNRNWR VCALMSCAKH KDYPFHAEV. . It is sometimes possible for the material contained within the vial of "Aldose Reductase, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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