UCH-L1 active protein

UCH-L1 (PGP9.5) (human), (recombinant) (His-tag)

Cat. Num. AAA566741

• Gene Names: UCHL1; PARK5; PGP95; PGP9.5; Uch-L1; PGP 9.5
• Synonyms: UCH-L1; UCH-L1 (PGP9.5) (human); (recombinant) (His-tag); UCH-L1 active protein

• Host: E Coli
• Form/Format: Liquid. In 50 mM TRIS, pH7.6, containing 1 mM dithiothreitol (DTT)

• Specific Activity: >100 pmol/min/ug. Determined at 25 degree C with 1uM ubiquitin-AMC as substrate and UCH-L1
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: For long term storage, store at -80 degree C; Shipping: Shipped on Dry Ice

Related Product Information for UCH-L1 active protein

Ubiquitin C-terminal hydrolases (UCHs) are a family of cysteine hydrolases that catalyze the hydrolysis of amides, esters and thioesters of the C-terminus of ubiquitin. Mammalian neuronal cells abundantly express a deubiquitylating enzyme, ubiquitin carboxy-terminal hydrolase 1 (UCH-L1). Mutations in UCH-L1 are linked to Parkinson's disease as well as gracile axonal dystrophy (gad) in mice. In contrast to the universally expressed UCH-L3 isozyme, UCH-L1 is expressed exclusively in neurons and testis/ovary. It has been shown that UCH-L1 associates and co-localizes with monoubiquitin and elongates ubiquitin half-life and the suggestion is made that UCH-L1, with avidity and affinity for ubiquitin, ensures ubiquitin stability within neurons.

Product Categories/Family for UCH-L1 active protein

Cellular Analysis; Stem Cells; Proteostasis; Protein Synthesis & Folding; Posttranslational Modifications; Ubiquitin & UBL Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 21361091
• NCBI GeneID: 7345
• NCBI Accession #: NP_004172.2
• NCBI GenBank Nucleotide #: NM_004181.4
• UniProt Accession #: P09936; Q4W5K6; Q71UM0
• Molecular Weight: 24,824 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L1
• NCBI Official Synonym Full Names: ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• NCBI Official Symbol: UCHL1
• NCBI Official Synonym Symbols: PARK5; PGP95; PGP9.5; Uch-L1; PGP 9.5
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin thioesterase L1; neuron cytoplasmic protein 9.5; ubiquitin C-terminal hydrolase
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L1
• UniProt Gene Name: UCHL1
• UniProt Synonym Gene Names: UCH-L1; PGP9.5
• UniProt Entry Name: UCHL1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]

Uniprot Description

UCHL1: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Monomer. Homodimer. Interacts with SNCA. Interacts with COPS5. Found in neuronal cell bodies and processes throughout the neocortex. Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. Belongs to the peptidase C12 family.

Protein type: Cell development/differentiation; EC 3.4.19.12; Ligase; Protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; cell soma; axon; cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; omega peptidase activity; ubiquitin binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; alpha-2A adrenergic receptor binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; cell proliferation; negative regulation of MAP kinase activity; protein deubiquitination; axon transport of mitochondrion; axon target recognition; eating behavior; neuromuscular process; sensory perception of pain; muscle fiber development; adult walking behavior

Disease: Neurodegeneration With Optic Atrophy, Childhood-onset; Parkinson Disease 5, Autosomal Dominant