Tumor Necrosis Factor Ligand Superfamily Member 10 (TNFSF10) Active Protein | TNFSF10 active protein

Recombinant Human Tumor Necrosis Factor Ligand Superfamily Member 10 (TNFSF10), Partial

Cat. Num. AAA7115087

• Gene Names: TNFSF10; TL2; APO2L; CD253; TRAIL; Apo-2L; TNLG6A
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Tumor Necrosis Factor Ligand Superfamily Member 10 (TNFSF10); Recombinant Human Tumor Necrosis Factor Ligand Superfamily Member 10 (TNFSF10); Partial; Tumor Necrosis Factor Ligand Superfamily Member 10; Apo-2 Ligand; Apo-2L; TNF-Related Apoptosis-Inducing Ligand; Protein TRAIL; CD253; TNFSF10; APO2L; TRAIL; TNFSF10 active protein

• Host: E Coli
• Specificity: Widespread; most predominant in spleen, lung and prostate.
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum Filtered 150 mM NaCl
• Sequence Positions: 115-281aa; Partial
• Sequence: RERGPQRVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSFLSNLHLRNGELVIHEKGFYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKYTSYPDPILLMKSARNSCWSKDAEYGLYSIYQGGIFELKENDRIFVSVTNEHLIDMDHEASFFGAFLVG
• Sequence Length: 101

• Species: Human
• Tag: Tag-Free
• Endotoxin: Less than 1.0 EU/ug as determined by LAL method.
• Biological Activity: The ED50 as determined in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D is less than 50 ng/ml.
• Subcellular Location: Membrane, Single-Pass Type II Membrane Protein
• Protein Families: Tumor Necrosis Factor Family
• Classification: Cytokine
• Subdivision: Tumor Necrosis Factor
• Pathway: Apoptosis
• Preparation and Storage: Store at -20 degree C. For extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for TNFSF10 active protein

Relevance: Human TNFSF10 is a type II transmembrane protein with an intracellular N-terminus and a 'TNF homology domain' (THD) at the extracellular C terminus. TNFSF10 can interact with several distinct receptors. Two of these receptors that belongs to TNFR superfamily, DR4 (TRAIL-R1) and DR5 (TRAIL-R2/TRICK2), are plasma membrane proteins containing intracellular death domains essential for activating apoptosis. TNFSF10 is promising for cancer therapy because it is cytotoxic and activates apoptosis in the majority of malignant cells, but not in normal cells.

Function: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG.

Product Categories/Family for TNFSF10 active protein

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 300193032
• NCBI GeneID: 8743
• NCBI Accession #: NP_001177871.1
• NCBI GenBank Nucleotide #: NM_001190942.1
• UniProt Accession #: P50591
• Molecular Weight: 19.5 kDa
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 10 isoform 2
• NCBI Official Synonym Full Names: TNF superfamily member 10
• NCBI Official Symbol: TNFSF10
• NCBI Official Synonym Symbols: TL2; APO2L; CD253; TRAIL; Apo-2L; TNLG6A
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 10
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 10
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: TNFSF10
• UniProt Synonym Gene Names: APO2L; TRAIL; Apo-2L
• UniProt Entry Name: TNF10_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

TRAIL: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. Homotrimer. Widespread; most predominant in spleen, lung and prostate. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q26

Cellular Component: extracellular space; integral to plasma membrane; extracellular region

Molecular Function: protein binding; metal ion binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; cell-cell signaling; positive regulation of apoptosis; apoptosis; male gonad development; positive regulation of caspase activity; immune response; signal transduction; response to insulin stimulus