TRAIL Active Protein | TNFSF10 active protein

TRAIL, Recombinant, Human (TNF-Related Apoptosis Inducing Ligand, Apo2L, TL2, TNFSF10)

Cat. Num. AAA650090

• Gene Names: TNFSF10; TL2; APO2L; CD253; TRAIL; Apo-2L
• Purity: Highly Purified; 97% (RP-HPLC, SDS-PAGE)
• Synonyms: TRAIL; Recombinant; Human (TNF-Related Apoptosis Inducing Ligand; Apo2L; TL2; TNFSF10); TNFSF10 active protein

• Host: E Coli
• Purity/Purification: Highly Purified; 97% (RP-HPLC, SDS-PAGE)
• Form/Format: Supplied as a lyophilized powder from 150mM sodium chloride, 50mM sodium phosphate, pH 7. Reconstitute with sterile ddH2O, 0.1% BSA or HSA to >0.1mg/ml.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Arg-Glu-Arg-Gly-Pro.

• Biological Activity: Recombinant Human TRAIL is fully biologically active when compared to standard. The activity is determined by the ability to induce apoptotic cell death in TRAIL-sensetive U343MG cells, ED50 for this effect is 1-3 ng/ml.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for TNFSF10 active protein

Recombinant Human soluble Trail also called TNF-related apoptosis-inducing Ligand, TNFSF10, Apo2 Ligand AND TL2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 168 amino acids and having a molecular mass of 19.6kD. TRAIL is purified by proprietary chromatographic techniques.

Product Categories/Family for TNFSF10 active protein

Growth Factors; Cytokines; Growth Factors-Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 4507593
• NCBI GeneID: 8743
• NCBI Accession #: NP_003801.1
• NCBI GenBank Nucleotide #: NM_003810.3
• UniProt Accession #: P50591; A1Y9B3
• Molecular Weight: 19.6kD
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 10 isoform 1
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 10
• NCBI Official Symbol: TNFSF10
• NCBI Official Synonym Symbols: TL2; APO2L; CD253; TRAIL; Apo-2L
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 10; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) family, member 10; chemokine tumor necrosis factor ligand superfamily member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 10
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: TNFSF10
• UniProt Synonym Gene Names: APO2L; TRAIL; Apo-2L
• UniProt Entry Name: TNF10_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

Function: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

Cofactor: Binds 1 zinc ion per trimer.

Subunit structure: Homotrimer.

Subcellular location: Membrane; Single-pass type II membrane protein

Potential.

Tissue specificity: Widespread; most predominant in spleen, lung and prostate.

Sequence similarities: Belongs to the tumor necrosis factor family.