TFF2 active protein

Recombinant Human TFF2

Cat. Num. AAA4159811

• Gene Names: TFF2; SP; SML1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: TFF2; Recombinant Human TFF2; Spasmolysin; Spasmolytic polypeptide; SP; Recombinant Human Trefoil Factor 2 (TFF2); TFF2 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered solution in PBS
• Sequence Length: 129

• Bioactivity: The ED(50) was determined by the dose-dependent proliferation of immortalized human pancreatic cells and was found to be <5ng/mL.
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method
• Family: Other growth factors/cytokines
• Reconstitution: Reconstitute at 100 ug/mL in sterile PBS
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Testing Data

Testing Data

Related Product Information for TFF2 active protein

Found in the gastrointestinal tract, the three Trefoil proteins, TFF1, 2, and 3 have been shown to play an important role in intestinal mucosal defence and repair. Specifically, TFF2 has been shown to inhibit gastrointestinal motility and secretion of the gastric acid.

NCBI and Uniprot Product Information

• NCBI GI #: 4885629
• NCBI GeneID: 7032
• NCBI Accession #: NP_005414.1
• NCBI GenBank Nucleotide #: NP_005414.1
• UniProt Accession #: Q03403
• Molecular Weight: 12.3 kDa
• NCBI Official Full Name: trefoil factor 2
• NCBI Official Synonym Full Names: trefoil factor 2
• NCBI Official Symbol: TFF2
• NCBI Official Synonym Symbols: SP; SML1
• NCBI Protein Information: trefoil factor 2
• UniProt Protein Name: Trefoil factor 2
• Protein Family: Trefoil factor
• UniProt Gene Name: TFF2
• UniProt Synonym Gene Names: SML1; SP
• UniProt Entry Name: TFF2_HUMAN

NCBI Description

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. The encoded protein inhibits gastric acid secretion. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]

Uniprot Description

TFF2: Inhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: extracellular space

Molecular Function: protein binding; CXCR4 chemokine receptor binding

Biological Process: negative regulation of inflammatory response; positive regulation of cell proliferation; digestion; calcium-mediated signaling; negative regulation of macrophage activation; regulation of cell migration