Prolactin Active Protein
Pegylated chicken prolactin
Purity
Greater than 99.0% as determined by: (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel.
Synonyms
Prolactin; Pegylated chicken prolactin; Prolactin active protein
Host
E Coli
Purity/Purification
Greater than 99.0% as determined by: (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel.
Form/Format
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02-0.03% NaHCO3.
Sequence
The amino terminal sequence could not be determined as the N- terminal amino acid was covalently bound to polyethylene glycol.
Physical Appearance
White lyophilized (freeze-dried) powder.
Solubility
It is recommended to reconstitute the lyophilized pegylated chPRL in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Dimers and Aggregates
The purified mono-pegylated chPRL consists of > 95% monomers as determined by gel-filtration chromatography.
Biological Activity
Pegylated chPRL is biologically active as evidenced by inducing proliferation of Nb2 cells or Baf/3 cells stably transfected with chPRL receptors, though its activity is lesser than hPRL. However it is anticipated that its activity in vivo will be higher than chPRL due to prolonged persistence in circulation.
Endotoxin
Less than 0.1 ng/µg (IEU/µg) of pegylated hPRL
Protein Content
Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 1.15 as the extinction coefficient for a 0.1% (1mg of protein part/ml) solution at pH 8.0. This value is calculated by the DNAman computer analysis program of protein sequences.
Preparation and Storage
Lyophilized pegylated hPRL although stable at room temperature for several weeks, should be stored desiccated below -18C. Upon reconstitution at > 0.1 pegylated chPRL (protein part) mg/ml and up to 4 mg/ml and filter sterilization pegylated hPRL can be stored at 4C for several weeks.
Related Product Information for Prolactin active protein
Recombinant chicken prolactin, consists of one polypeptide chain containing 199 amino and an additional Ala at N-terminus acids was mono-pegylated and purified by proprietary chromatographic techniques as described by Oclon et al. (in press). Its molecular mass is ~ 39 kDa, however under non-denaturing conditions it behaves as 220 kDa protein due to its increased hydrodynamic volume.
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Product Notes
The Prolactin (Catalog #AAA400134) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The amino terminal sequence could not be determined as the N- terminal amino acid was covalently bound to polyethyle ne glycol. It is sometimes possible for the material contained within the vial of "Prolactin, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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