PPA1 active protein

PPA1, human recombinant

Cat. Num. AAA844780

• Gene Names: PPA1; PP; PP1; IOPPP; HEL-S-66p; SID6-8061
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: PPA1; human recombinant; Inorganic pyrophosphatase; IOPPP; PP; PP1; SID6-8061; PPA1 active protein

• Host: E Coli
• Purity/Purification: >=95%
• Form/Format: 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 200 mM NaCl and 2 mM DTT; Appearance: Liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSGFST EERAAPFSLE YRVFLKNEKG QYISPFHDIP IYADKDVFHM VVEVPRWSNA KMEIATKDPL NPIKQDVKKG KLRYVANLFP YKGYIWNYGA IPQTWEDPGH NDKHTGCCGD NDPIDVCEIG SKVCARGEII GVKVLGILAM IDEGETDWKV IAINVDDPDA ANYNDINDVK RLKPGYLEAT VDWFRRYKVP DGKPENEFAF NAEFKDKDFA IDIIKSTHDH WKALVTKKTN GKGISCMNTT LSESPFKCDP DAARAIVDAL PPPCESACTV PTDVDKWFHH QKN
• Sequence Length: 289

• Applicable Applications for PPA1 active protein: SDS-PAGE
• Biological Activity: Specific activity is >28,000 pmoles/min/ug, and measured by its ability to hydrolyze pyrophosphate per minute at pH 9.0 at 25 degree C
• Results: Specific activity is >28,000 pmoles/min/ug
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for PPA1 active protein

Background: PPA1 (Pyrophosphatase) belongs to the PPase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. The hydrolysis of inorganic pyrophosphate (PPi) to two phosphate ions is utilized in many biochemical pathways to render reactions effectively irreversible. Inorganic pyrophosphatase catalyzes this hydrolysis reaction in the early steps of lipid degradation, a prominent example of this phenomenon. By promoting the rapid hydrolysis of pyrophosphate (PPi), Inorganic pyrophosphatase provides the driving force for the activation of fatty acids destined for oxidation. Recombinant human PPA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Product Categories/Family for PPA1 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 11056044
• NCBI GeneID: 5464
• NCBI Accession #: NP_066952.1
• NCBI GenBank Nucleotide #: NM_021129.3
• UniProt Accession #: Q15181; Q2M348; Q5SQT7; Q6P7P4; Q9UQJ5; Q9Y5B1
• Molecular Weight: 35.2 kDa (313 aa, 1-289 aa + His Tag)
• NCBI Official Full Name: inorganic pyrophosphatase
• NCBI Official Synonym Full Names: pyrophosphatase (inorganic) 1
• NCBI Official Symbol: PPA1
• NCBI Official Synonym Symbols: PP; PP1; IOPPP; HEL-S-66p; SID6-8061
• NCBI Protein Information: inorganic pyrophosphatase
• UniProt Protein Name: Inorganic pyrophosphatase
• Protein Family: Soluble inorganic pyrophosphatase
• UniProt Gene Name: PPA1
• UniProt Synonym Gene Names: IOPPP; PP; PPase
• UniProt Entry Name: IPYR_HUMAN

NCBI Description

The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme. [provided by RefSeq, Jul 2008]

Uniprot Description

PPA1: is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme. [provided by RefSeq, Jul 2008]

Protein type: Energy Metabolism - oxidative phosphorylation; Hydrolase; Motility/polarity/chemotaxis; EC 3.6.1.1

Chromosomal Location of Human Ortholog: 10q11.1-q24

Cellular Component: cytoplasm; cytosol

Molecular Function: inorganic diphosphatase activity; magnesium ion binding

Biological Process: tRNA aminoacylation for protein translation; gene expression; phosphate metabolic process