Platelet Derived Growth Factor CC Active Protein | PDGFC active protein

Platelet Derived Growth Factor CC, Recombinant, Human (PDGF-CC)

Cat. Num. AAA651088

• Gene Names: PDGFC; SCDGF; FALLOTEIN
• Purity: >98% by SDS-PAGE and HPLC analyses.
• Synonyms: Platelet Derived Growth Factor CC; Recombinant; Human (PDGF-CC); PDGFC active protein

• Host: E Coli
• Purity/Purification: >98% by SDS-PAGE and HPLC analyses.
• Form/Format: Supplied as a lyophilized powder from 5mM sodium citrate , pH 3.0
• Sequence: MVVDLNLLTE EVRLYSCTPR NFSVSIREEL KRTDTIFWPG CLLVKRCGGN CACCLHNCNE CQCVPSKVTK KYHEVLQLRP KTGVRGLHKS LTDVALEHHE ECDCVCRGST GG

• Reconstitution: Reconstitute with sterile ddH2O to 0.1-1 mg/ml. Do not vortex.
• Endotoxin: <0.1 ng/ug (1EU/ug)
• Biological Activity: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected The ED50 for this effect is 15-20ng/ml.
• Preparation and Storage: Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH20. Aliquot to avoid repeated freezing and thawing . Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for PDGFC active protein

The platelet-derived growth factor (PDGF) family of heparin-binding growth factors consists of five known members, denoted PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC and PDGF-DD. The mature and active form of these proteins, an anti-parallel disulfide-linked dimer of two 12-14kD polypeptide chains, is obtained through proteolytic processing of biologically inactive precursor proteins, which contain an N-terminal CUB domain and a PDGF/VEGF homologous domain. The PDGFs interact with two related protein tyrosine kinase receptors, PDGFR- and PDGFR-, and are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and certain tumor cells. They play an important role in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubules epithelial cell development. Mature PDGFs are stored in platelet -granules and are released upon platelet activation. PDGF-AA, -AB, -BB and -CC signal primarily through the PDGF-R receptor, whereas PDGF-DD interacts almost exclusively with the PDGF-R receptor. Recombinant human PDGF-CC is a 25kD protein consisting of two identical disulfide-linked 114 amino-acid polypeptide chains.

Product Categories/Family for PDGFC active protein

Growth Factors; Cytokines; Growth Factors-PDGF

NCBI and Uniprot Product Information

• NCBI GI #: 9994187
• NCBI GeneID: 56034
• NCBI Accession #: NP_057289.1
• NCBI GenBank Nucleotide #: NM_016205.2
• UniProt Accession #: Q9NRA1; Q4W5M9; Q9UL22; B4DU34; B9EGR8
• NCBI Official Full Name: platelet-derived growth factor C
• NCBI Official Synonym Full Names: platelet derived growth factor C
• NCBI Official Symbol: PDGFC
• NCBI Official Synonym Symbols: SCDGF; FALLOTEIN
• NCBI Protein Information: platelet-derived growth factor C; PDGF-C; VEGF-E; spinal cord-derived growth factor; secretory growth factor-like protein
• UniProt Protein Name: Platelet-derived growth factor C
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: PDGFC
• UniProt Synonym Gene Names: SCDGF; PDGF-C; SCDGF; PDGFC latent form
• UniProt Entry Name: PDGFC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines. This gene product appears to form only homodimers. It differs from the platelet-derived growth factor alpha and beta polypeptides in having an unusual N-terminal domain, the CUB domain. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2010]

Uniprot Description

Function: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen and chemoattractant for cells of mesenchymal origin. Required for normal skeleton formation during embryonic development, especially for normal development of the craniofacial skeleton and for normal development of the palate. Required for normal skin morphogenesis during embryonic development. Plays an important role in wound healing, where it appears to be involved in three stages: inflammation, proliferation and remodeling. Plays an important role in angiogenesis and blood vessel development. Involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintenance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function. Ref.2 Ref.3 Ref.4 Ref.14 Ref.15 Ref.18 Ref.19 Ref.22 Ref.23 Ref.24 Ref.25 Ref.27

Subunit structure: Homodimer; disulfide-linked. Interacts with PDGFRA homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts (via CUB domain) with PLAT (via kringle domain). Ref.17 Ref.19 Ref.22

Subcellular location: Cytoplasm. Secreted. Nucleus. Cytoplasmic granule. Note: Sumoylated form is predominant in the nucleus. Stored in alpha granules in platelets. Membrane associated when bound to receptors. Ref.2 Ref.3 Ref.4 Ref.18 Ref.19 Ref.22 Ref.26

Tissue specificity: Expressed in the fallopian tube, vascular smooth muscle cells in kidney, breast and colon and in visceral smooth muscle of the gastrointestinal tract. Highly expressed in retinal pigment epithelia. Expressed in medulloblastoma. In the kidney, constitutively expressed in parietal epithelial cells of Bowman's capsule, tubular epithelial cells and in arterial endothelial cells (at protein level). Highly expressed in the platelets, prostate, testis and uterus. Higher expression is observed in uterine leiomyomata. Weaker expression in the spleen, thymus, heart, pancreas, liver, ovary cells and small intestine, and negligible expression in the colon and peripheral blood leukocytes. Ref.1 Ref.3 Ref.4 Ref.11 Ref.13 Ref.14 Ref.18 Ref.27 Ref.28

Developmental stage: In the fetal kidney, detected in the developing mesangium, ureteric bud epithelium and the undifferentiated mesenchyme (at protein level). Ref.16

Induction: Up-regulated by EWS-FLI1 chimeric transcription factor in tumor derived cells. Up-regulated in podocytes and interstitial cells after injury/activation of these cells. FGF2 activates PDGFC transcription via EGR1. Up-regulated by TGFB1 in concert with FGF2. Ref.12 Ref.16 Ref.20 Ref.25

Post-translational modification: Proteolytic removal of the N-terminal CUB domain releasing the core domain is necessary for unmasking the receptor-binding epitopes of the core domain. Cleavage after basic residues in the hinge region (region connecting the CUB and growth factor domains) gives rise to the receptor-binding form. Cleaved by PLAT and PLG.Sumoylated with SUMO1. Ref.26N-glycosylated. Ref.14

Miscellaneous: A lower molecular weight form (around 43 kDa) is present in patients with papillary thyroid carcinoma.

Sequence similarities: Belongs to the PDGF/VEGF growth factor family.Contains 1 CUB domain.