PGK1 active protein

PGK1, human recombinant

Cat. Num. AAA844637

• Gene Names: PGK1; PGKA; MIG10; HEL-S-68p
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: PGK1; human recombinant; Phosphoglycerate kinase 1; MGC117307; MGC142128; MGC8947; MIG10; PGKA; PGK1 active protein

• Host: E Coli
• Purity/Purification: >=95%
• Form/Format: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, and 1 mM DTT; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MSLSNKLTLD KLDVKGKRVV MRVDFNVPMK NNQITNNQRI KAAVPSIKFC LDNGAKSVVL MSHLGRPDGV PMPDKYSLEP VAVELKSLLG KDVLFLKDCV GPEVEKACAN PAAGSVILLE NLRFHVEEEG KGKDASGNKV KAEPAKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPQKAGGFLM KKELNYFAKA LESPERPFLA ILGGAKVADK IQLINNMLDK VNEMIIGGGM AFTFLKVLNN MEIGTSLFDE EGAKIVKDLM SKAEKNGVKI TLPVDFVTAD KFDENAKTGQ ATVASGIPAG WMGLDCGPES SKKYAEAVTR AKQIVWNGPV GVFEWEAFAR GTKALMDEVV KATSRGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKVLPG VDALSNI
• Sequence Length: 417

• Applicable Applications for PGK1 active protein: SDS-PAGE
• Biological Activity: Specific activity is >190 units/mg One unit will convert 1.0 umole of beta-NADH per minute in a coupled system with GAPDH at pH 8.0 at 25 degree C
• Results: Specific activity is >190 units/mg
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for PGK1 active protein

Background: Phosphoglycerate kinase 1 (PGK1) is an X-linked enzyme that plays a key role in the glycolytic pathway. The gene encoding the erythrocyte enzyme PGK1 and it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) as a glyxolytic enzyme role. The PGK1 catalyzes the reversible conversion of 1, 3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. Recombinant PGK1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Product Categories/Family for PGK1 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 4505763
• NCBI GeneID: 5230
• NCBI Accession #: NP_000282.1
• NCBI GenBank Nucleotide #: NM_000291.3
• UniProt Accession #: P00558; Q5J7W1; Q6IBT6; Q8NI87; A8K4W6; B7Z7A9
• Molecular Weight: 46.8 kDa (437 aa, 1-417 aa + His Tag)
• NCBI Official Full Name: phosphoglycerate kinase 1
• NCBI Official Synonym Full Names: phosphoglycerate kinase 1
• NCBI Official Symbol: PGK1
• NCBI Official Synonym Symbols: PGKA; MIG10; HEL-S-68p
• NCBI Protein Information: phosphoglycerate kinase 1
• UniProt Protein Name: Phosphoglycerate kinase 1
• Protein Family: Phosphoglycerate kinase
• UniProt Gene Name: PGK1
• UniProt Synonym Gene Names: PGKA; PRP 2
• UniProt Entry Name: PGK1_HUMAN

NCBI Description

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014]

Uniprot Description

PGK1: an apparent multifunctional protein. A glycolytic enzyme and a polymerase alpha cofactor protein (primer recognition protein).

Protein type: EC 2.7.2.3; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other

Chromosomal Location of Human Ortholog: Xq13.3

Cellular Component: membrane; cytosol

Molecular Function: protein binding; phosphoglycerate kinase activity; ATP binding

Biological Process: glycolysis; epithelial cell differentiation; carbohydrate metabolic process; glucose metabolic process; pathogenesis; phosphorylation; gluconeogenesis

Disease: Phosphoglycerate Kinase 1 Deficiency