PGD active protein

PGD Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120246

• Gene Names: PGD; 6PGD
• Purity: >88% as determined by SDS-PAGE
• Synonyms: PGD; PGD Protein; Human; Recombinant (His Tag); Human PGD/Phosphogluconate dehydrogenase Protein (His Tag); 6PGD Protein; phosphogluconate dehydrogenase; PGD active protein

• Host: E Coli
• Purity/Purification: >88% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala483

• Species: Human
• Activity: Measured by its ability to dehydrogenate 6phosphogluconic acid. The specific activity is >2500 pmols/min/ug
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human PGD (P52209)(Met 1-Ala 483) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

References

Baillet A, et al. (2011) Coupling of 6-phosphogluconate dehydrogenase with NADPH oxidase in neutrophils: Nox2 activity regulation by NADPH availability. FASEB J. 25(7): 2333-43.Rho HK, et al. (2005) Transcriptional regulation of mouse 6-phosphogluconate dehydrogenase by ADD1/SREBP1c. Biochem Biophys Res Commun. 332(1): 288-96.Broedel SE, et al. (1990). Genetic tagging, cloning, and DNA sequence of the Synechococcus sp. strain PCC 7942 gene (gnd) encoding 6-phosphogluconate dehydrogenase. J Bacteriol. 172 (7): 4023-31.Adams MJ, et al. (1983). The three dimensional structure of sheep liver 6-phosphogluconate dehydrogenase at 2.6 A resolution. EMBO J. 2 (6): 1009-14.

NCBI and Uniprot Product Information

• NCBI GI #: 40068518
• NCBI GeneID: 5226
• NCBI Accession #: NP_002622.2
• NCBI GenBank Nucleotide #: NM_002631.2
• UniProt Accession #: P52209; Q9BWD8; A8K2Y9
• Molecular Weight: 53,140 Da
• NCBI Official Full Name: 6-phosphogluconate dehydrogenase, decarboxylating
• NCBI Official Synonym Full Names: phosphogluconate dehydrogenase
• NCBI Official Symbol: PGD
• NCBI Official Synonym Symbols: 6PGD
• NCBI Protein Information: 6-phosphogluconate dehydrogenase, decarboxylating
• UniProt Protein Name: 6-phosphogluconate dehydrogenase, decarboxylating
• UniProt Gene Name: PGD
• UniProt Synonym Gene Names: PGDH
• UniProt Entry Name: 6PGD_HUMAN

NCBI Description

6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies. [provided by RefSeq, Jul 2008]

Uniprot Description

PGD: an oxidative carboxylase that catalyses the decarboxylating reduction of 6-phosphogluconate into ribulose 5-phosphate in the presence of NADP. This reaction is a component of the hexose mono-phosphate shunt and pentose phosphate pathways.

Protein type: Oxidoreductase; Carbohydrate Metabolism - pentose phosphate pathway; Other Amino Acids Metabolism - glutathione; EC 1.1.1.44

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: nucleus; cytosol

Molecular Function: phosphogluconate dehydrogenase (decarboxylating) activity; NADP binding

Biological Process: pentose-phosphate shunt; pentose-phosphate shunt, oxidative branch; carbohydrate metabolic process; pathogenesis; D-gluconate metabolic process; pentose biosynthetic process