Pegylated Leptin Active Protein | Lep active protein

Recombinant Pegylated Mouse Leptin

Cat. Num. AAA143325

• Gene Names: Lep; ob; obese
• Purity: Greater than 99.0% as determined by: (a) Analysis by Gel-Filtration. (b) Analysis by SDS-PAGE.
• Synonyms: Pegylated Leptin; Recombinant Pegylated Mouse Leptin; Leptin Mouse; PEG; Pegylated Mouse Leptin Recombinant; OB Protein; Obesity Protein; OBS; Obesity factor; mLeptin PEG; Lep active protein

• Host: E Coli
• Purity/Purification: Greater than 99.0% as determined by: (a) Analysis by Gel-Filtration. (b) Analysis by SDS-PAGE.
• Form/Format: The mouse Leptin was lyophilized from a concentrated (0.65mg/ml) solution containing 0.003mM NaHCO3.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence Length: 167

• Solubility: It is recommended to reconstitute the lyophilized Leptin in sterile 0.4% NaHCO3 adjusted to pH-8.5 not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: Pegylated mouse Leptin is capable of stimulatng proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Pegylated mouse Leptin in vitro activity is only slightly lower than the non-pegylated antagonist but in vivo it has profound weight reducing effect (as compared to the non-pegylated leptin), resulting mainly from reduced food intake.
• Preparation and Storage: Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Leptin should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for Lep active protein

Description: Leptin Mono-Pegylated Mouse Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and an additional Ala at N-terminus. Pegylated Mouse Leptin contains PEG 20 kDa at its N-terminus and having a molecular mass of 35.6 kDa as determined by mass spectrometry. Since its enlarged hydrodymanic volume Pegylated Leptin runs on SDS-PAGE as A 48 kDa protein and in gel-filtration on Superdex 200 as over 100 kDa protein. Pegylated Mouse Leptin half-life in circulation after SC injection was over 20 hours. Mouse Leptin was purified by proprietary chromatographic techniques according to Salomon et al (2006) Protein Expression and Purification 47, 128136 and then pegylated.

Introduction: A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.

Product Categories/Family for Lep active protein

Cytokines and Growth Factors; Growth Factors; Leptin

NCBI and Uniprot Product Information

• NCBI GI #: 6678678
• NCBI GeneID: 16846
• NCBI Accession #: NP_032519.1
• NCBI GenBank Nucleotide #: NM_008493.3
• UniProt Accession #: P41160
• Molecular Weight: 18,709 Da
• NCBI Official Full Name: leptin
• NCBI Official Synonym Full Names: leptin
• NCBI Official Symbol: Lep
• NCBI Official Synonym Symbols: ob; obese
• NCBI Protein Information: leptin; obesity factor
• UniProt Protein Name: Leptin
• Protein Family: Leptin
• UniProt Gene Name: Lep
• UniProt Synonym Gene Names: Ob
• UniProt Entry Name: LEP_MOUSE

Uniprot Description

leptin: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in LEP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the leptin family.

Protein type: Secreted; Secreted, signal peptide; Hormone; Cell development/differentiation

Cellular Component: extracellular space; cell; cytoplasm; extracellular region; intracellular

Molecular Function: protein binding; growth factor activity; peptide hormone receptor binding; hormone activity

Biological Process: response to dietary excess; positive regulation of myeloid cell differentiation; regulation of fat cell differentiation; positive regulation of JAK-STAT cascade; regulation of steroid biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; signal transduction; negative regulation of metabolic process; negative regulation of appetite; positive regulation of tyrosine phosphorylation of Stat3 protein; response to insulin stimulus; positive regulation of MAPKKK cascade; regulation of cholesterol absorption; regulation of blood pressure; positive regulation of cell proliferation; positive regulation of ion transport; regulation of metabolic process; placenta development; central nervous system neuron development; cholesterol metabolic process; regulation of protein amino acid phosphorylation; positive regulation of developmental growth; eating behavior; bile acid metabolic process; glucose metabolic process; adult feeding behavior; fatty acid catabolic process; ovulation from ovarian follicle; leptin-mediated signaling pathway; negative regulation of vasoconstriction; tyrosine phosphorylation of STAT protein; fatty acid beta-oxidation; positive regulation of protein import into nucleus; insulin secretion; glycerol biosynthetic process; energy reserve metabolic process; hormone metabolic process; regulation of gluconeogenesis; positive regulation of insulin receptor signaling pathway; lipid metabolic process; leukocyte tethering or rolling; regulation of insulin secretion; negative regulation of apoptosis