Ostreolysin Active Protein
Ostreolysin (cytotisin from Pleorotus ostreatus)
Purity
Greater than 98.0% as determined by:(a) Analysis by reducing and non-reducing SDS-PAGE gel.
(b) Gel-filtration chromatography under non denaturing conditions.
Synonyms
Ostreolysin; Ostreolysin (cytotisin from Pleorotus ostreatus); Ostreolysin active protein
Host
E Coli
Purity/Purification
Greater than 98.0% as determined by:
(a) Analysis by reducing and non-reducing SDS-PAGE gel.
(b) Gel-filtration chromatography under non denaturing conditions.
(a) Analysis by reducing and non-reducing SDS-PAGE gel.
(b) Gel-filtration chromatography under non denaturing conditions.
Form/Format
White powder
Sequence
The N-terminal amino sequence is Ala-Tyr-Ala-Gln-Trp-Val
Sequence Length
137
Solubility
It is recommended to reconstitute the lyophilized ostreolysin in sterile 0.4% NaHCO3 adjusted, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content
Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 2.64 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the DNA-man computer analysis program.
Dimers and Aggregates
Less than 2%.
Endotoxin
Less than 0.1 ng/ug (IEU/ug) of ostreolysin
Formulation
Ostreolysin was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3.
Biological Activity
Ostreolysin has potent anti-carcinogenic activity in several colon cancer cell lines.
Preparation and Storage
Lyophilized ostreolysin although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution at > 0.1 A-protein mg/ml and up to 2 mg and filter sterilization ostreolysin can be stored at +4 degree C.
Related Product Information for Ostreolysin active protein
Ostreolysin, is a cytosolic prtein of 15 kDa pore-forming protein from the edible oyster mushroom (Pleurotus ostreatus), is lytic to membranes containing both cholesterol and sphingomyelin. Its cytotoxicity to Chinese hamster ovary cells correlates with their cholesterol contents and with the occurrence of ostreolysin in the cells detergent resistant membranes. Moreover, ostreolysin binds to supported monolayers and efficiently permeabilizes sonicated lipid vesicles, only if cholesterol is combined with either sphingomyelin or dipalmitoyl-phosphatidylcholine. Addition of mono- or di-unsaturated phosphatidylcholine to the cholesterol/sphingomyelin vesicles dramatically reduces the ostreolysin's activity. It appears that the protein recognizes specifically a cholesterol-rich lipid phase, probably the liquid-ordered phase. Ostreolysin was purified by combination of ion-exchange and size-exclusion chromatography.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
14,855 Da
NCBI Official Full Name
ostreolysin, partial
UniProt Protein Name
Ostreolysin
Protein Family
UniProt Entry Name
Q56QW9_PLEOS
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Product Notes
The Ostreolysin (Catalog #AAA400123) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The N-terminal amino sequence is Ala-Tyr-Al a-Gln-Trp- Val. It is sometimes possible for the material contained within the vial of "Ostreolysin, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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