Ostreolysin Active Protein
Recombinant Pleurotus Ostreatus Ostreolysin
Purity
>98.0% as determined by:(a) Analysis by Gel filtration analysis.
(b) Analysis by SDS-PAGE.
Synonyms
Ostreolysin; Recombinant Pleurotus Ostreatus Ostreolysin; Ostreolysin Pleurotus Ostreatus Recombinant; Ostreolysin active protein
Host
E Coli
Purity/Purification
>98.0% as determined by:
(a) Analysis by Gel filtration analysis.
(b) Analysis by SDS-PAGE.
(a) Analysis by Gel filtration analysis.
(b) Analysis by SDS-PAGE.
Form/Format
Sterile filtered white lyophilized (freeze-dried) powder.
The Ostreolysin protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3
The Ostreolysin protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3
Sequence
Amino acid: N-terminal amino sequence: Ala-Tyr-Ala-Gln-Trp-Val.
Sequence Length
137
Solubility
It is recommended to reconstitute the lyophilized Ostreolysin in sterile 0.4% NaHCO3, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
Ostreolysin has potent anti-carcinogenic activity in several colon cancer cell lines.
Protein Content
Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 2.64 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the DNA-man computer analysis program.
Preparation and Storage
Lyophilized Pleurotus Ostreatus Ostreolysin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon C between 2-7 days and for future use reconstituted Ostreolysin should be stored at 4 degree C below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Please avoid freeze-thaw cycles.
Related Product Information for Ostreolysin active protein
Introduction: Ostreolysin is extracted from Pleurotus ostreatus (oyster mushroom). It is a pore forming protein, which contains a lytic part to both cholesterol and sphingomyelin containing membranes. Because of their cholesterol content and the appearance of ostreolysin in the detergent resistant membranes, ostreolysin is cytotoxic towards the ovary cells of Chinese hamster. It seems that Ostreolysin spots a rich lipid cholesterol phase, presumably the liquid ordered phase.
Description: Pleurotus Ostreatus Ostreolysin Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 137 amino acids and having a molecular mass of 15 kDa.The Ostreolysin is purified by proprietary chromatographic techniques.
Description: Pleurotus Ostreatus Ostreolysin Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 137 amino acids and having a molecular mass of 15 kDa.The Ostreolysin is purified by proprietary chromatographic techniques.
Product Categories/Family for Ostreolysin active protein
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
NCBI Official Full Name
Ostreolysin
Protein Family
Similar Products
Product Notes
The Ostreolysin (Catalog #AAA141478) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Amino acid: N-terminal amino sequence: Ala-Tyr-Al a-Gln-Trp- Val. It is sometimes possible for the material contained within the vial of "Ostreolysin, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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