Nodal active protein

Recombinant Human Nodal

Cat. Num. AAA4159858

• Gene Names: NODAL; HTX5
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Nodal; Recombinant Human Nodal; Nodal homolog; Nodal active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered solution in Acetonitrile and TFA (with BSA as a carrier protein)
• Sequence Length: 347

• Bioactivity: 100 ng/mL phosphorylation activity as determined by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method
• Reconstitution: Reconstitute in sterile 4mM HCl at 100ug/mL (containing at least 0.1% human or bovine serum albumin)
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Testing Data

Related Product Information for Nodal active protein

Nodal, also known as Nodal homolog, is a secreted protein member of the Transforming Growth Factor-beta (TGF-beta) superfamily. It is essential for the formation of mesoderm and axial patterning during early vertebrate embryonic development.

NCBI and Uniprot Product Information

• NCBI GI #: 222352098
• NCBI GeneID: 4838
• NCBI Accession #: NP_060525.3
• NCBI GenBank Nucleotide #: NP_060525.3
• UniProt Accession #: Q96S42
• Molecular Weight: 13.1 kDa
• NCBI Official Full Name: nodal homolog isoform 1 preproprotein
• NCBI Official Synonym Full Names: nodal growth differentiation factor
• NCBI Official Symbol: NODAL
• NCBI Official Synonym Symbols: HTX5
• NCBI Protein Information: nodal homolog
• UniProt Protein Name: Nodal homolog
• Protein Family: Nodal
• UniProt Gene Name: NODAL
• UniProt Entry Name: NODAL_HUMAN

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which regulates early embryonic development. This protein is required for maintenance of human embryonic stem cell pluripotency and may play a role in human placental development. Mutations in this gene are associated with heterotaxy, a condition characterized by random orientation of visceral organs with respect to the left-right axis. [provided by RefSeq, Aug 2016]

Uniprot Description

NODAL: Essential for mesoderm formation and axial patterning during embryonic development. Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5). A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia. Belongs to the TGF-beta family.

Protein type: Cytokine; Cell development/differentiation; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: extracellular space

Molecular Function: morphogen activity; growth factor activity; cytokine activity; receptor agonist activity; transforming growth factor beta receptor binding

Biological Process: embryonic placenta development; mesendoderm development; positive regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; axial mesodermal cell fate specification; embryonic pattern specification; positive regulation of activin receptor signaling pathway; floor plate morphogenesis; vasculature development; regulation of gastrulation; positive regulation of cell-cell adhesion; trophectodermal cellular morphogenesis; heart looping; cell migration involved in gastrulation; maternal placenta development; placenta development; embryonic process involved in female pregnancy; stem cell maintenance; digestive tract morphogenesis; embryonic cranial skeleton morphogenesis; liver development; formation of anatomical boundary; neural fold formation; positive regulation of angiogenesis; repression of premature neural plate formation; polarity specification of proximal/distal axis; maternal process involved in parturition; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; brain development; positive regulation of epithelial cell proliferation; growth

Disease: Heterotaxy, Visceral, 5, Autosomal