NMNAT2 active protein

NMNAT2, human recombinant

Cat. Num. AAA844361

• Gene Names: NMNAT2; PNAT2; C1orf15
• Purity: > 85% by SDS-PAGE
• Synonyms: NMNAT2; human recombinant; Nicotinamide mononucleotide Adenylyltransferase 2; C1orf15; PNAT2; NMNAT2 active protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: 0.25 mg/ml solution 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 150 mM NaCl and 1 mM DTT.; Appearance: Liquid
• Sequence: MGSSHHHHHH SSGLVPRGSH MTETTKTHVI LLACGSFNPI TKGHIQMFER ARDYLHKTGR FIVIGGIVSP VHDSYGKQGL VSSRHRLIMC QLAVQNSDWI RVDPWECYQD TWQTTCSVLE HHRDLMKRVT GCILSNVNTP SMTPVIGQPQ NETPQPIYQN SNVATKPTAA KILGKVGESL SRICCVRPPV ERFTFVDENA NLGTVMRYEE IELRILLLCG SDLLESFCIP GLWNEADMEV IVGDFGIVVV PRDAADTDRI MNHSSILRKY KNNIMVVKDD INHPMSVVSS TKSRLALQHG DGHVVDYLSQ PVIDYILKSQ LYINASG
• Sequence Length: 307

• Biological Activity: Specific activity: > 500 pmol/min/ug. One unit will convert 1.0 pmole of beta-NADH per minute to beta-NAD at PH 8.0 at 37 degree C
• Preparation and Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Related Product Information for NMNAT2 active protein

NMNAT2 belongs to the nicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family, members of which catalyze an essential step in NAD (NADP) biosynthetic pathway. Unlike the other human family member, which is localized to the nucleus, and is ubiquitously expressed; this enzyme is cytoplasmic, and is predominantly expressed in the brain. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human NMNAT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Product Categories/Family for NMNAT2 active protein

Diabetes; Obesity & Metabolic Syndrome; Lipids Metabolism; Proteins/Enzymes; OtherProteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 24307989
• NCBI GeneID: 23057
• NCBI Accession #: NP_055854.1
• NCBI GenBank Nucleotide #: NM_015039.3
• UniProt Accession #: Q9BZQ4; O75067; Q5T1Q3; Q8WU99; Q96QW1
• Molecular Weight: 36.6 kDa (327 aa, 1-307 aa + His Tag)
• NCBI Official Full Name: nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2 isoform 1
• NCBI Official Synonym Full Names: nicotinamide nucleotide adenylyltransferase 2
• NCBI Official Symbol: NMNAT2
• NCBI Official Synonym Symbols: PNAT2; C1orf15
• NCBI Protein Information: nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2
• UniProt Protein Name: Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 2
• UniProt Gene Name: NMNAT2
• UniProt Synonym Gene Names: C1orf15; KIAA0479; NMN/NaMN adenylyltransferase 2; NMN adenylyltransferase 2; NaMN adenylyltransferase 2
• UniProt Entry Name: NMNA2_HUMAN

NCBI Description

This gene product belongs to the nicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family, members of which catalyze an essential step in NAD (NADP) biosynthetic pathway. Unlike the other human family member, which is localized to the nucleus, and is ubiquitously expressed; this enzyme is cytoplasmic, and is predominantly expressed in the brain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

NMNAT2: Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency. Cannot use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity prefers NAD(+), NADH and NAAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+). Belongs to the eukaryotic NMN adenylyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.7.18; EC 2.7.7.1; Transferase; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: Golgi membrane; Golgi apparatus; late endosome; synapse; trans-Golgi network

Molecular Function: nicotinamide-nucleotide adenylyltransferase activity; nicotinate-nucleotide adenylyltransferase activity; ATP binding

Biological Process: NAD biosynthetic process; vitamin metabolic process; NAD metabolic process; water-soluble vitamin metabolic process