MMP-12 active protein

MMP-12 (human), (recombinant) (catalytic domain)

Cat. Num. AAA566572

• Purity: >=95% (SDS-PAGE); Purified by multi-step chromatography
• Synonyms: MMP-12; MMP-12 (human); (recombinant) (catalytic domain); MMP-12 active protein

• Host: E Coli
• Purity/Purification: >=95% (SDS-PAGE); Purified by multi-step chromatography
• Form/Format: Liquid. In 50mM TRIS, pH9.5, containing 5mM calcium chloride, 500mM sodium chloride, 20uM zinc chloride, 0.5% Brij-35, and 30% glycerol

• Source Note: Active Matrix Metalloproteinase-12 (MMP-12, metalloelastase, macrophage elastase; often confused with neutrophil elastase) catalytic domain from human cDNA. The enzyme consists of the catalytic domain of human MMP-12 (Phe99-Leu271) with a C-terminal purification tag
• Activity: Preincubation of MMP-12 catalytic domain at 4nM with the inhibitor NNGH at 20nM, or with the broad-spectrum inhibitor GM6001 at 5nM, for 1 hour completely inhibits enzymatic activity
• Specific Activity: >=30U/ug. One U=100 pmol/min at 37 degree C using the colorimetric thiopeptolide Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-OEt (100uM) as substrate
• Product Notes: In an SDS-PAGE gel, the enzyme runs as a doublet (~20 kDa). The higher band represents the polypeptide described above, while spontaneous cleavage of the tag results in the lower band. Both species possess identical enzymatic activities
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: For long term storage, store at -80 degree C; The enzyme is stable on ice for at least several hours. However, it is recommended that thawing and dilution of the enzyme be done within as short a time as possible before start of the assay. When stored per recommendation, this enzyme is stable at the concentration supplied, in its current storage buffer. Procedures such as dilution of the enzyme followed by refreezing could lead to loss of activity; Shipping: Shipped on Dry Ice

Product Categories/Family for MMP-12 active protein

Cellular Analysis; Cell Organelles & Structures; Proteostasis; Proteolysis & Protein Degradation

NCBI and Uniprot Product Information

• UniProt Accession #: P39900
• Molecular Weight: 203kDa
• UniProt Protein Name: Macrophage metalloelastase
• UniProt Gene Name: MMP12
• UniProt Synonym Gene Names: HME; MME; ME; hME; MMP-12
• UniProt Entry Name: MMP12_HUMAN

Uniprot Description

MMP12: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. Belongs to the peptidase M10A family.

Protein type: Secreted, signal peptide; Secreted; EC 3.4.24.65; Protease

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular region

Molecular Function: zinc ion binding; metalloendopeptidase activity; endopeptidase activity; calcium ion binding

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; positive regulation of epithelial cell proliferation involved in wound healing; proteolysis; wound healing, spreading of epidermal cells