MHC Class 1 Chain-related Gene B Active Protein

MHC Class 1 Chain-related Gene B, Recombinant, Human (MICB)

Cat. Num. AAA637846

• Purity: Highly Purified; 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
• Synonyms: MHC Class 1 Chain-related Gene B; Recombinant; Human (MICB); MHC Class 1 Chain-related Gene B active protein

• Host: E Coli
• Purity/Purification: Highly Purified; 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.
• Form/Format: Supplied as a lyophilized powder without additives.

• Biological Activity: Recombinant Human MICB is fully biologically active when compared to standards. Measured by its ability to bind MICB antibody in ELISA.
• Dimers and Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
• Endotoxin Level: 0.1ng/ug (IEU/ug) of Recombinant Human MICB.
• Protein Content: Protein quantitation was carried out by two independent methods:; 1. UV spectroscopy at 280 nm.; 2. Analysis by RP-HPLC, using a standard solution of MICB as a Reference Standard.
• Solubility: It is recommended to reconstitute the lyophilized MICB in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: -20 degree C; Stability: Lyophilized Recombinant Human MICB although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution MICB should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Related Product Information for MHC Class 1 Chain-related Gene B active protein

MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB. These 2 proteins are distantly related to the MHC class I proteins. MICA and MICB (MICA/B) possess three extracellular immunoglobulin-like domains, but have no capacity to bind peptide or interact with 2-microglobulin. The genes encoding MICA/B are found within the major histocompatibility complex on human chromosome 6. The MICB locus is polymorphic with more than 15 recognized human alleles. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B are ligands for NKG2D, an activating receptor expressed on NK cells, NKT cells, T cells, and CD8+ T cells. Recognition of MICA/B by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MICA/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response.

rHuMICB produced in E.Coli is a single, non-glycosylated polypeptide chain containing 326 amino acids and having a molecular mass of 37kD. The sequence contains the extracellular domain of the mature human MICB (amino acid residues Ala23 - Tyr312) MICB is purified by proprietary chromatographic techniques.

Product Categories/Family for MHC Class 1 Chain-related Gene B active protein

Molecular Biology; MB-Carbohydrates; Glycoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 302310538
• Molecular Weight: 37kD
• NCBI Official Full Name: MHC class I chain-related protein B, partial

NCBI Description

This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]