MCAF Active Protein

MCAF, Recombinant, Human (MCP-1, Macrophage/Monocyte Chemotactic and Activating Factor)

Cat. Num. AAA650850

• Purity: Highly Purified; 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
• Synonyms: MCAF; Recombinant; Human (MCP-1; Macrophage/Monocyte Chemotactic and Activating Factor); MCAF active protein

• Host: E Coli
• Purity/Purification: Highly Purified; 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
• Form/Format: Supplied as a lyophilized powder. No additives.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ala-Ile.

• Biological Activity: MCP-1 is fully biologically active when compared to standard. The specific activity as determined by the ability of MCP-1 to chemoattract human Monocytes using a concentration of 5-20ng/ml.
• Dimers and Aggregates: 1% as determined by silver-stained SDS-PAGE gel analysis.
• Endotoxin Level: 0.1ng/ug (IEU/ug) of rHuMCP-1.
• Protein Content: Protein quantitation was carried out by two independent methods:; 1. UV spectroscopy at 280nm.; 2. Analysis by RP-HPLC, using a standard solution of MCP-1 as a Reference Standard.
• Reconstitution: Reconstitute the lyophilized rHuMCP-1 in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for MCAF active protein

Recombinant Human MCP-1 also known as Monocyte Chemotactic and Activating Factor (MCAF) is a non-glycosylated, Polypeptide chain containing 76 amino acids and having a molecular mass of 8607 Dalton.

Product Categories/Family for MCAF active protein

Growth Factors; Cytokines; Growth Factors-MCAF

NCBI and Uniprot Product Information

• NCBI GI #: 29611342
• NCBI Official Full Name: MBD1-containing chromatin associated factor

NCBI Description

ATF7IP is a multifunctional nuclear protein that associates with heterochromatin. It can act as a transcriptional coactivator or corepressor depending upon its binding partners (summary by Liu et al., 2009 [PubMed 19106100]).[supplied by OMIM, Nov 2010]

Uniprot Description

ATF7IP: Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. Required to stimulate histone methyltransferase activity of SETDB1 and facilitate the conversion of dimethylated to trimethylated H3 'Lys-9' (H3K9me3). The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3). Facilitates telomerase TERT and TERC gene expression by SP1 in cancer cells. Belongs to the MCAF family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 12p13.1

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: protein binding; ATPase activity; transcription corepressor activity

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; viral reproduction; positive regulation of transcription, DNA-dependent; DNA methylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of transcriptional preinitiation complex assembly