Listeriolysin O, PEST-free Active Protein

Listeriolysin O, PEST-free, Recombinant (LLO)

Purity

Highly Purified
90% as determined by:

Synonyms

Listeriolysin O; PEST-free; Recombinant (LLO); PEST-free active protein

Ordering
Cat. Num. AAA636027 When autocomplete results are available use up and down arrows to review and enter to select. Touch device users, explore by touch or with swipe gestures. For Research Use Only!

Host

E Coli

Purity/Purification

Highly Purified
90% as determined by:

Form/Format

Supplied as a liquid in 50mM NaH2PO4, 1mM EDTA, 2.7mM potassium chloride, 1mM DTT, 5% glycerol and 0.5M sodium chloride

Application Notes

a. Cytosolic delivery of molecules, peptides, oligonucleodides and plasmid DNA (4). b. Production of specific monoclonal antibodies (5). c. Detection of anti-listeriolysin O antibodies (6).

Activity

~1.25x106 HU/mg. 2 mM DTT could be use to reactivate the toxin.

Endotoxin Level

<0.1ng/ug (IEU/ug) of LLO.

Preparation and Storage

-20 degree C
Stability: rLLO although stable at 140C for 1 week, should be stored desiccated below -180C. Please prevent freeze-thaw cycles. Please avoid freeze-thaw cycles.

Related Product Information for Listeriolysin O, PEST-free active protein

The secreted pore-forming protein listeriolysin O (LLO) is an essential virulence determinant of the facultative intracellular bacterium Listeria monocytogenes that allows the pathogen to escape from the phagocytic vacuole and reach the host cytosol. LLO is a single polypeptide protein encoded by the hlyA gene and composed of 529 residues. At its NH2-terminus it possesses a 25 residues long typical signal sequence excited during the secretion process. Moreover, in its NH2-terminus there is also a 19 amino acids PEST-like sequence that may target this toxin for degradation. This motif seems to be essential for bacterial virulence too, since its deletion causes a defect in bacterium phagosomal escaping.

Recombinant Listeriolysin O lacking both the signal secretion sequence and the PEST- like sequence. LLO-PEST minus is composed of 471 amino acids, starting from amino acid 60 to amino acid 529, with the addition of a methionine in its NH2-terminus.

Product Categories/Family for Listeriolysin O, PEST-free active protein

Molecular Biology; MB-Proteins

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Product Notes

The Listeriolysin O, PEST-free (Catalog #AAA636027) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. a. Cytosolic delivery of molecules, peptides, oligonucleodides and plasmid DNA (4). b. Production of specific monoclonal antibodies (5). c. Detection of anti-listeriolysin O antibodies (6). Researchers should empirically determine the suitability of the Listeriolysin O, PEST-free for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Listeriolysin O, PEST-free, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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